BMRB Entry 16954

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PDB ID: 2kyg
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16954
MolProbity Validation Chart

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Title:
Structure of the AML1-ETO Nervy Domain - PKA(RIIa) complex and its contribution to AML1-ETO activity
Deposition date:
2010-05-25
Original release date:
2010-08-19
Authors:
Corpora, Takeshi; Cierpecki, Tomasz; Bushweller, John
Citation:

Citation: Corpora, Takeshi; Roudaia, Liya; Oo, Zaw Min; Chen, Wei; Manuylova, Ekaterina; Cai, Xiongwei; Chen, Michael; Cierpicki, Tomasz; Speck, Nancy; Bushweller, John. "Structure of the AML1-ETO NHR3-PKA(RII) Complex and Its Contribution to AML1-ETO Activity."  J. Mol. Biol. 402, 560-577 (2010).
PubMed: 20708017

Assembly members:

Assembly members:
PKA(RIIa), polymer, 50 residues, 5621.476 Da.
NHR3, polymer, 38 residues, 4184.758 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHis Parallel 2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PKA(RIIa): GAMGSMSHIQIPPGLTELLQ GYTVEVLRQQPPDLVEFAVE YFTRLREARA
NHR3: AMADIGSASGYVPEEIWKKA EEAVNEVKRQAMTELQKA

Data sets:
Data typeCount
13C chemical shifts470
15N chemical shifts134
1H chemical shifts897

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PKA(RIIa) 11
2PKA(RIIa) 21
3NHR32

Entities:

Entity 1, PKA(RIIa) 1 50 residues - 5621.476 Da.

GAMGSMS is a vector artifact from the pHis-Parallel2 expression vector.

1   GLYALAMETGLYSERMETSERHISILEGLN
2   ILEPROPROGLYLEUTHRGLULEULEUGLN
3   GLYTYRTHRVALGLUVALLEUARGGLNGLN
4   PROPROASPLEUVALGLUPHEALAVALGLU
5   TYRPHETHRARGLEUARGGLUALAARGALA

Entity 2, NHR3 38 residues - 4184.758 Da.

AMADIGS is a vector artifact from the pET-32a expression vector.

1   ALAMETALAASPILEGLYSERALASERGLY
2   TYRVALPROGLUGLUILETRPLYSLYSALA
3   GLUGLUALAVALASNGLUVALLYSARGGLN
4   ALAMETTHRGLULEUGLNLYSALA

Related Database Links:

PDB 2HWN 2IZX 2KYG
DBJ BAF85731 BAG73608 BAK63879 BAA03089 BAA03247 BAA03558 BAA06774 BAA07755
EMBL CAA33094 CAA67817 CAA56311 CAG33073
GB AAH02763 AAM97689 AAP35889 AAP36931 AAX29035 AAB34819 AAC28931 AAC28932 AAH05850 AAH67078
PRF 1506340A
REF NP_001266877 NP_004148 NP_062137 XP_003894514 XP_005075011 NP_001102127 NP_001104496 NP_001104497 NP_001185554 NP_001185555
SP P12368 P13861 Q06455 Q61909
AlphaFold P12368 P13861 Q06455 Q61909

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks