BMRB Entry 16947

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PDB ID: 2ksd
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16947
MolProbity Validation Chart

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Title:
Backbone structure of the membrane domain of E. coli histidine kinase receptor ArcB
Deposition date:
2010-05-24
Original release date:
2010-06-15
Authors:
Maslennikov, Innokentiy; Klammt, Christian; Hwang, Eunha; Kefala, Georgia; Kwiatkowski, Witek; Jeon, Young Ho; Choe, Senyon
Citation:

Citation: Maslennikov, Innokentiy; Klammt, Christian; Hwang, Eunha; Kefala, Georgia; Okamura, Mizuki; Esquivies, Luis; Mors, Karsten; Glaubitz, Clemens; Kwiatkowski, Witek; Jeon, Young Ho; Choe, Senyon. "Membrane domain structures of three classes of histidine kinase receptors by cell-free expression and rapid NMR analysis."  Proc. Natl. Acad. Sci. U.S.A. 107, 10902-10907 (2010).
PubMed: 20498088

Assembly members:

Assembly members:
ArcB, polymer, 115 residues, 8483.369 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: pIVEX2.3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ArcB: MKQIRLLAQYYVDLMMKLGL VRFSMLLALALVVLAIVVQM AVTMVLHGQVESIDVIRSIF FGLLITPWAVYFLSVVVEQL EESRQRLSRLVQKLEEMRER DLSLNVQLKDNIAQL

Data sets:
Data typeCount
13C chemical shifts317
15N chemical shifts108
1H chemical shifts491

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1ArcB1

Entities:

Entity 1, ArcB 115 residues - 8483.369 Da.

The membrane domain of ArcB

1   METLYSGLNILEARGLEULEUALAGLNTYR
2   TYRVALASPLEUMETMETLYSLEUGLYLEU
3   VALARGPHESERMETLEULEUALALEUALA
4   LEUVALVALLEUALAILEVALVALGLNMET
5   ALAVALTHRMETVALLEUHISGLYGLNVAL
6   GLUSERILEASPVALILEARGSERILEPHE
7   PHEGLYLEULEUILETHRPROTRPALAVAL
8   TYRPHELEUSERVALVALVALGLUGLNLEU
9   GLUGLUSERARGGLNARGLEUSERARGLEU
10   VALGLNLYSLEUGLUGLUMETARGGLUARG
11   ASPLEUSERLEUASNVALGLNLEULYSASP
12   ASNILEALAGLNLEU

Related Database Links:

PDB 2KSD
DBJ BAB37512 BAE77254 BAG79018 BAH65394 BAI27488
EMBL CAA37397 CAD07845 CAP77670 CAQ33543 CAQ90680
GB AAA58012 AAG58344 AAL22197 AAN44715 AAN82410
PIR AG0906
REF NP_312116 NP_457707 NP_462238 NP_709008 WP_000809745
SP P0AEC3 P0AEC4 P58363
AlphaFold P0AEC3 P0AEC4 P58363

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks