BMRB Entry 16920

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PDB ID: 2kxn
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16920
MolProbity Validation Chart

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Title:
NMR structure of human Tra2beta1 RRM in complex with AAGAAC RNA
Deposition date:
2010-05-10
Original release date:
2011-03-24
Authors:
Clery, Antoine; Benderska, Natalya; Jayne, Sandrine; Dominguez, Cyril; Stamm, Stefan; Allain, Frederic
Citation:

Citation: Clery, Antoine; Jayne, Sandrine; Benderska, Natalya; Dominguez, Cyril; Stamm, Stefan; Allain, Frederic H-T. "Molecular basis of purine-rich RNA recognition by the human SR-like protein Tra2-1."  Nat. Struct. Mol. Biol. 18, 443-450 (2011).
PubMed: 21399644

Assembly members:

Assembly members:
RNA, polymer, 6 residues, 1922.268 Da.
Tra2beta1, polymer, 95 residues, 10944.354 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA: AAGAAC
Tra2beta1: RHVGNRANPDPNCCLGVFGL SLYTTERDLREVFSKYGPIA DVSIVYDQQSRRSRGFAFVY FENVDDAKEAKERANGMELD GRRIRVDFSITKRPH

Data sets:
Data typeCount
13C chemical shifts280
15N chemical shifts92
1H chemical shifts653

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA (5'-R(*AP*AP*GP*AP*AP*C)-3')1
2Tra2beta12

Entities:

Entity 1, RNA (5'-R(*AP*AP*GP*AP*AP*C)-3') 6 residues - 1922.268 Da.

1   AAGAAC

Entity 2, Tra2beta1 95 residues - 10944.354 Da.

1   ARGHISVALGLYASNARGALAASNPROASP
2   PROASNCYSCYSLEUGLYVALPHEGLYLEU
3   SERLEUTYRTHRTHRGLUARGASPLEUARG
4   GLUVALPHESERLYSTYRGLYPROILEALA
5   ASPVALSERILEVALTYRASPGLNGLNSER
6   ARGARGSERARGGLYPHEALAPHEVALTYR
7   PHEGLUASNVALASPASPALALYSGLUALA
8   LYSGLUARGALAASNGLYMETGLULEUASP
9   GLYARGARGILEARGVALASPPHESERILE
10   THRLYSARGPROHIS

Related Database Links:

BMRB 11400 11409 11410 11411 11412 11413
PDB 2CQC 2KXN 2RRA 2RRB
DBJ BAA08556 BAC05256 BAC33819 BAD92445 BAE88784
EMBL CAA56518 CAH18071 CAH90742
GB AAB08701 AAC28242 AAD19277 AAD19278 AAG35783
REF NP_001006878 NP_001029948 NP_001070689 NP_001125414 NP_001230808
SP P62995 P62996 P62997 Q3ZBT6
TPG DAA33375
AlphaFold P62995 P62996 P62997 Q3ZBT6

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SPARKY: Backbone or all simulated peaks