BMRB Entry 16919

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16919

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Title:
Backbone 1H,13C, and 15N assignments of Streptococcus pneumoniae mevalonate diphosphate decarboxylase (spMDD) in apo-state
Deposition date:
2010-05-10
Original release date:
2010-09-08
Authors:
Reuther, Guido; Harris, Richard; Girvin, Mark; Leyh, Thomas
Citation:

Citation: Reuther, Guido; Harris, Richard; Girvin, Mark; Leyh, Thomas. "Backbone (1)H, (13)C, (15)N NMR assignments of the unliganded and substrate ternary complex forms of mevalonate diphosphate decarboxylase from Streptococcus pneumoniae."  Biomol. NMR Assignments 5, 11-14 (2011).
PubMed: 20737255

Assembly members:

Assembly members:
mevalonate_diphosphate_decarboxylase, polymer, 317 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: purified from the natural source   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mevalonate_diphosphate_decarboxylase: MDREPVTVRSYANIAIIKYW GKKKEKEMVPATSSISLTLE NMYTETTLSPLPANVTADEF YINGQLQNEVEHAKMSKIID RYRPAGEGFVRIDTQNNMPT AAGLSSSSSGLSALVKACNA YFKLGLDRSQLAQEAKFASG SSSRSFYGPLGAWDKDSGEI YPVETDLKLAMINLVLEDKK KPISSRDGMKLCVETSTTFD DWVRQSEKDYQDMLIYLKEN DFAKIGELTEKNALAMHATT KTASPAFSYLTDASYEAMDF VRQLREKGEACYFTMDAGPN VKVFCQEKDLEHLSEIFGQR YRLIVSKTKDLSQDDCC

Data sets:
Data typeCount
13C chemical shifts832
15N chemical shifts268
1H chemical shifts268

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1spMDD1

Entities:

Entity 1, spMDD 317 residues - Formula weight is not available

1   METASPARGGLUPROVALTHRVALARGSER
2   TYRALAASNILEALAILEILELYSTYRTRP
3   GLYLYSLYSLYSGLULYSGLUMETVALPRO
4   ALATHRSERSERILESERLEUTHRLEUGLU
5   ASNMETTYRTHRGLUTHRTHRLEUSERPRO
6   LEUPROALAASNVALTHRALAASPGLUPHE
7   TYRILEASNGLYGLNLEUGLNASNGLUVAL
8   GLUHISALALYSMETSERLYSILEILEASP
9   ARGTYRARGPROALAGLYGLUGLYPHEVAL
10   ARGILEASPTHRGLNASNASNMETPROTHR
11   ALAALAGLYLEUSERSERSERSERSERGLY
12   LEUSERALALEUVALLYSALACYSASNALA
13   TYRPHELYSLEUGLYLEUASPARGSERGLN
14   LEUALAGLNGLUALALYSPHEALASERGLY
15   SERSERSERARGSERPHETYRGLYPROLEU
16   GLYALATRPASPLYSASPSERGLYGLUILE
17   TYRPROVALGLUTHRASPLEULYSLEUALA
18   METILEASNLEUVALLEUGLUASPLYSLYS
19   LYSPROILESERSERARGASPGLYMETLYS
20   LEUCYSVALGLUTHRSERTHRTHRPHEASP
21   ASPTRPVALARGGLNSERGLULYSASPTYR
22   GLNASPMETLEUILETYRLEULYSGLUASN
23   ASPPHEALALYSILEGLYGLULEUTHRGLU
24   LYSASNALALEUALAMETHISALATHRTHR
25   LYSTHRALASERPROALAPHESERTYRLEU
26   THRASPALASERTYRGLUALAMETASPPHE
27   VALARGGLNLEUARGGLULYSGLYGLUALA
28   CYSTYRPHETHRMETASPALAGLYPROASN
29   VALLYSVALPHECYSGLNGLULYSASPLEU
30   GLUHISLEUSERGLUILEPHEGLYGLNARG
31   TYRARGLEUILEVALSERLYSTHRLYSASP
32   LEUSERGLNASPASPCYSCYS

Samples:

sample_1: mevalonate diphosphate decarboxylase, [U-100% 13C; U-100% 15N; U-80% 2H], 1 ± 0.05 mM; HEPES 25 ± 0.05 mM; potassium chloride 50 ± 0.1 mM; DTT 1.6 ± 0.01 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, data analysis, processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 16916
EMBL CAR68209 CBW32038 CBW33977 CBW36013 CCM08953
GB AAG02456 AAK74549 AAK99143 ABJ54071 ACA35923
REF NP_357933 WP_000373447 WP_000373452 WP_000373453 WP_000373454

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks