BMRB Entry 16908

Assembly:

Entities:

Entity 1, UBLCP1 81 residues - 8997.747 Da.

Samples:

sample_1: UBLdomain of UBLCP1, [U-99% 15N], 1.5 mM; H2O 90%; D2O 10%; HEPES 10 mM; NaCl 100 mM

sample_2: UBLdomain of UBLCP1, [U-99% 13C; U-99% 15N], 1.5 mM; H2O 90%; D2O 10%; HEPES 10 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

CYANA v2.2.5, P.GUNTERT ET AL. - structure solution

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

Molmol, Koradi, Billeter and Wuthrich - data analysis

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

ProcheckNMR, Laskowski and MacArthur - geometry optimization

SPARKY, Goddard - chemical shift assignment

xwinnmr, Bruker Biospin - collection

NMR spectrometers:

• Bruker DRX 900 MHz
• Bruker DRX 500 MHz