BMRB Entry 16892

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16892
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Title:
NS2 [60-99]
Deposition date:
2010-04-22
Original release date:
2011-05-19
Authors:
Montserret, Roland; Bartenschlager, Ralf; Penin, Francois
Citation:

Citation: Jirasko, Vlastimil; Montserret, Roland; Lee, Ji Young; Gouttenoire, Jerome; Moradpour, Darius; Penin, Francois; Bartenschlager, Ralf. "Structural and functional studies of nonstructural protein 2 of the hepatitis C virus reveal its key role as organizer of virion assembly."  PLoS Pathog. 6, .-. (2010).
PubMed: 21187906

Assembly members:

Assembly members:
NS2[60-99], polymer, 40 residues, 4275.335 Da.

Natural source:

Natural source:   Common Name: Hepatitis C Virus   Taxonomy ID: 11103   Superkingdom: virus   Kingdom: not available   Genus/species: Hepacivirus Hepatitis C Virus

Experimental source:

Experimental source:   Production method: chemical synthesis   Host organism: not applicable

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NS2[60-99]: GRDAVILLTCAIHPELIFTI TKILLAILGPLMVLQAGITK

Data sets:
Data typeCount
1H chemical shifts297

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NS2[60-99]1

Entities:

Entity 1, NS2[60-99] 40 residues - 4275.335 Da.

1   GLYARGASPALAVALILELEULEUTHRCYS
2   ALAILEHISPROGLULEUILEPHETHRILE
3   THRLYSILELEULEUALAILELEUGLYPRO
4   LEUMETVALLEUGLNALAGLYILETHRLYS

Samples:

sample_1: NS2[60-99] 0.4 mM; TFE, [U-2H], 50 % v/v; H2O 50 % v/v; DSS 0.2 uM

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

SPARKY, Goddard - data analysis

X-PLOR NIH v2.24, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

PDB
EMBL CAB46677 CAB46911 CAB46915 CAB53095
GB ABV46075 ABV46118 ABV46121 ABV46124 ABV46226
SP Q9WMX2
AlphaFold Q9WMX2