BMRB Entry 16840

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PDB ID: 2l9c
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16840
MolProbity Validation Chart

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Title:
1H, 15N and 13C resonance assignment of Darcin, a Major Urinary Protein of the mouse
Deposition date:
2010-04-06
Original release date:
2010-08-19
Authors:
Phelan, Marie; McLean, Lynn; Simpson, Deborah; Hurst, Jane; Beynon, Robert; Lian, Lu-Yun
Citation:

Citation: Phelan, Marie; McLean, Lynn; Simpson, Deborah; Hurst, Jane; Beynon, Robert; Lian, Lu-Yun. "1H, 15N and 13C resonance assignment of darcin, a mouse major urinary protein."  Biomol. NMR Assignments 4, 239-241 (2010).
PubMed: 20703836

Assembly members:

Assembly members:
darcin, polymer, 176 residues, 20533.9058 Da.

Natural source:

Natural source:   Common Name: House mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
darcin: MGSSHHHHHHIEGREEASSM ERNFNVEKINGEWYTIMLAT DKREKIEEHGSMRVFVEYIH VLENSLALKFHIIINEECSE IFLVADKTEKAGEYSVTYDG SNTFTILKTDYDNYIMIHLI NKKDGETFQLMELYGREPDL SSDIKEKFAQLSEEHGIVRE NIIDLTNANRCLEARE

Data sets:
Data typeCount
13C chemical shifts781
15N chemical shifts186
1H chemical shifts1202

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1darcin1

Entities:

Entity 1, darcin 176 residues - 20533.9058 Da.

residues 20 to 181 correspond to the native MUP residues 6 to 19 correspond to the cloning and purification tag

1   METGLYSERSERHISHISHISHISHISHIS
2   ILEGLUGLYARGGLUGLUALASERSERMET
3   GLUARGASNPHEASNVALGLULYSILEASN
4   GLYGLUTRPTYRTHRILEMETLEUALATHR
5   ASPLYSARGGLULYSILEGLUGLUHISGLY
6   SERMETARGVALPHEVALGLUTYRILEHIS
7   VALLEUGLUASNSERLEUALALEULYSPHE
8   HISILEILEILEASNGLUGLUCYSSERGLU
9   ILEPHELEUVALALAASPLYSTHRGLULYS
10   ALAGLYGLUTYRSERVALTHRTYRASPGLY
11   SERASNTHRPHETHRILELEULYSTHRASP
12   TYRASPASNTYRILEMETILEHISLEUILE
13   ASNLYSLYSASPGLYGLUTHRPHEGLNLEU
14   METGLULEUTYRGLYARGGLUPROASPLEU
15   SERSERASPILELYSGLULYSPHEALAGLN
16   LEUSERGLUGLUHISGLYILEVALARGGLU
17   ASNILEILEASPLEUTHRASNALAASNARG
18   CYSLEUGLUALAARGGLU

Related Database Links:

PDB 2L9C
EMBL CAP58483 CAQ11567
GB AAH89613 AAH92096 ACF70718
REF NP_001012323 XP_006538108
SP Q5FW60
TPG DAA06315
AlphaFold Q5FW60

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