BMRB Entry 16811

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PDB ID: 2kw8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16811
MolProbity Validation Chart

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Title:
Solution Structure of Bacillus anthracis Sortase A (SrtA) Transpeptidase
Deposition date:
2010-03-31
Original release date:
2010-05-28
Authors:
Weiner, Ethan; Robson, Scott; Marohn, Melanie; Clubb, Robert
Citation:

Citation: Weiner, Ethan; Robson, Scott; Marohn, Melanie; Clubb, Robert. "The Sortase A enzyme that attaches proteins to the cell wall of Bacillus anthracis contains an unusual active site architecture."  J. Biol. Chem. 285, 23433-23443 (2010).
PubMed: 20489200

Assembly members:

Assembly members:
SrtA, polymer, 158 residues, 17110.521 Da.

Natural source:

Natural source:   Common Name: Bacillus anthracis   Taxonomy ID: 1392   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus anthracis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SrtA: GSHMDASKIDQPDLAEVANA SLDKKQVIGRISIPSVSLEL PVLKSSTEKNLLSGAATVKE NQVMGKGNYALAGHNMSKKG VLFSDIASLKKGDKIYLYDN ENEYEYAVTGVSEVTPDKWE VVEDHGKDEITLITCVSVKD NSKRYVVAGDLVGTKAKK

Data sets:
Data typeCount
13C chemical shifts592
15N chemical shifts146
1H chemical shifts962

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SrtA1

Entities:

Entity 1, SrtA 158 residues - 17110.521 Da.

Residues 52-56 represent a non-native affinity tag

1   GLYSERHISMETASPALASERLYSILEASP
2   GLNPROASPLEUALAGLUVALALAASNALA
3   SERLEUASPLYSLYSGLNVALILEGLYARG
4   ILESERILEPROSERVALSERLEUGLULEU
5   PROVALLEULYSSERSERTHRGLULYSASN
6   LEULEUSERGLYALAALATHRVALLYSGLU
7   ASNGLNVALMETGLYLYSGLYASNTYRALA
8   LEUALAGLYHISASNMETSERLYSLYSGLY
9   VALLEUPHESERASPILEALASERLEULYS
10   LYSGLYASPLYSILETYRLEUTYRASPASN
11   GLUASNGLUTYRGLUTYRALAVALTHRGLY
12   VALSERGLUVALTHRPROASPLYSTRPGLU
13   VALVALGLUASPHISGLYLYSASPGLUILE
14   THRLEUILETHRCYSVALSERVALLYSASP
15   ASNSERLYSARGTYRVALVALALAGLYASP
16   LEUVALGLYTHRLYSALALYSLYS

Related Database Links:

BMRB 11570 26510
PDB 2KW8 2RUI
DBJ BAL16456 BAR78533 GAE96182 GAO57723 GAO63420
EMBL CJA38300 CJJ39486 CKE38654 CKE81514 CKE96545
GB AAP24701 AAS39688 AAT29792 AAT52982 AAU19641
REF NP_843215 WP_001041406 WP_001041710 WP_001041711 WP_001041715

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