BMRB Entry 16796

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16796

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Title:
Solution NMR structure of the Cdt1 binding domain(CBD) in complex with the MCM6 binding domain (MBD)
Deposition date:
2010-03-28
Original release date:
2011-05-19
Authors:
LIU, Changdong; WEI, Zhun; ZHU, Guang
Citation:

Citation: Wei, Zhun; Liu, Changdong; Wu, Xing; Xu, Naining; Zhou, Bo; Liang, Chun; Zhu, Guang. "Characterization and structure determination of the Cdt1 binding domain of human minichromosome maintenance (Mcm) 6."  J. Biol. Chem. 285, 12469-12473 (2010).
PubMed: 20202939

Assembly members:

Assembly members:
CBD, polymer, 114 residues, 13304.011 Da.
MBD, polymer, 30 residues, 3359.926 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CBD: APKASLRLGFSEYSRISNLI VLHLRKVEEEEDESALKRSE LVNWYLKEIESEIDSEEELI NKKRIIEKVIHRLTHYDHVL IELTQAGLKGSTEGSESYEE DPYLVVNPNYLLED
MBD: SPSALKGVSQDLLERIRAKE AQKQLAQMTR

Data sets:
Data typeCount
13C chemical shifts364
15N chemical shifts126
1H chemical shifts774

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CBD1
2MBD2

Entities:

Entity 1, CBD 114 residues - 13304.011 Da.

1   ALAPROLYSALASERLEUARGLEUGLYPHE
2   SERGLUTYRSERARGILESERASNLEUILE
3   VALLEUHISLEUARGLYSVALGLUGLUGLU
4   GLUASPGLUSERALALEULYSARGSERGLU
5   LEUVALASNTRPTYRLEULYSGLUILEGLU
6   SERGLUILEASPSERGLUGLUGLULEUILE
7   ASNLYSLYSARGILEILEGLULYSVALILE
8   HISARGLEUTHRHISTYRASPHISVALLEU
9   ILEGLULEUTHRGLNALAGLYLEULYSGLY
10   SERTHRGLUGLYSERGLUSERTYRGLUGLU
11   ASPPROTYRLEUVALVALASNPROASNTYR
12   LEULEUGLUASP

Entity 2, MBD 30 residues - 3359.926 Da.

1   SERPROSERALALEULYSGLYVALSERGLN
2   ASPLEULEUGLUARGILEARGALALYSGLU
3   ALAGLNLYSGLNLEUALAGLNMETTHRARG

Related Database Links:

BMRB 16396 17702 17702
PDB 2KLQ 2LE8 2WVR
DBJ BAA12699 BAE01174 BAG35469 BAG61268 BAB61878
GB AAB48165 AAC50766 AAH32374 AAI12449 AAO26043 AAG45181 AAH00137 AAH08676 AAH08860 AAH09410
REF NP_001039699 NP_001247544 NP_001270984 NP_005906 XP_001154732 NP_112190 XP_003280692 XP_004058184 XP_006926820 XP_008973138
SP Q14566 Q2KIZ8 Q9H211
AlphaFold Q14566 Q2KIZ8 Q9H211

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks