BMRB Entry 16775

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16775
MolProbity Validation Chart

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Title:
Mesencephalic astrocyte-derived neurotrophic factor (MANF)
Deposition date:
2010-03-12
Original release date:
2022-02-14
Authors:
Hellman, Maarit; Peranen, Johan; Saarma, Mart; Permi, Perttu
Citation:

Citation: Hellman, Maarit; Arumae, Urmas; Yu, Li-ying; Lindholm, Paivi; Peranen, Johan; Saarma, Mart; Permi, Perttu. "Mesencephalic astrocyte-derived neurotrophic factor (MANF) has a unique mechanism to rescue apoptotic neurons"  J. Biol. Chem. 286, 2675-2680 (2011).
PubMed: 21047780

Assembly members:

Assembly members:
MANF, polymer, 160 residues, 18339.395 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pFOLD

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MANF: GSLRPGDCEVCISYLGRFYQ DLKDRDVTFSPATIENELIK FCREARGKENRLCYYIGATD DAATKIINEVSKPLAHHIPV EKICEKLKKKDSQICELKYD KQIDLSTVDLKKLRVKELKK ILDDWGETCKGCAEKSDYIR KINELMPKYAPKAASARTDL

Data sets:
Data typeCount
13C chemical shifts568
15N chemical shifts160
1H chemical shifts1119

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MANF1

Entities:

Entity 1, MANF 160 residues - 18339.395 Da.

First two residues are cloning artefacts

1   GLYSERLEUARGPROGLYASPCYSGLUVAL
2   CYSILESERTYRLEUGLYARGPHETYRGLN
3   ASPLEULYSASPARGASPVALTHRPHESER
4   PROALATHRILEGLUASNGLULEUILELYS
5   PHECYSARGGLUALAARGGLYLYSGLUASN
6   ARGLEUCYSTYRTYRILEGLYALATHRASP
7   ASPALAALATHRLYSILEILEASNGLUVAL
8   SERLYSPROLEUALAHISHISILEPROVAL
9   GLULYSILECYSGLULYSLEULYSLYSLYS
10   ASPSERGLNILECYSGLULEULYSTYRASP
11   LYSGLNILEASPLEUSERTHRVALASPLEU
12   LYSLYSLEUARGVALLYSGLULEULYSLYS
13   ILELEUASPASPTRPGLYGLUTHRCYSLYS
14   GLYCYSALAGLULYSSERASPTYRILEARG
15   LYSILEASNGLULEUMETPROLYSTYRALA
16   PROLYSALAALASERALAARGTHRASPLEU

Samples:

sample_1: MANF, [U-98% 13C; U-98% 15N], 1 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 50 mM; pH: 6.8; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCANsample_1isotropicsample_conditions_1
3D HCA(CO)Nsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_1isotropicsample_conditions_1
2D (HB)CB(CGCDCE)HEsample_1isotropicsample_conditions_1

Software:

CYANA v2.0.26, P.GUNTERT ET AL. - automated NOE assignment

AMBER v8, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement, structure solution

NMR spectrometers:

  • Varian Unity Inova 600 MHz
  • Varian Unity Inova 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks