BMRB Entry 16769

Name: Solution structure of the par toxin Fst in DPC micelles
Published: 2010-08-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16769

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the par toxin Fst in DPC micelles

Deposition date:

2010-03-08

Original release date:

2010-08-12

Authors:

Zangger, Klaus; Gobl, Christoph; Kosol, Simone; Rueckert, Hanna Marie

Citation:

Citation: Gobl, Christoph; Kosol, Simone; Stockner, Thomas; Ruckert, Hanna; Zangger, Klaus. "Solution structure and membrane binding of the toxin fst of the par addiction module." Biochemistry 49, 6567-6575 (2010).
PubMed: 20677831

Assembly members:

Assembly members:
Fst, polymer, 33 residues, 3767.501 Da.

Natural source:

Natural source: Common Name: E. faecalis Taxonomy ID: 1351 Superkingdom: Bacteria Kingdom: not available Genus/species: Enterococcus faecalis

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Fst: MKDLMSLVIAPIFVGLVLEM ISRVLDEEDDSRK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	193

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Fst	1

Entities:

Entity 1, Fst 33 residues - 3767.501 Da.

1

MET

LYS

ASP

LEU

MET

SER

LEU

VAL

ILE

ALA

2

PRO

ILE

PHE

VAL

GLY

LEU

VAL

LEU

GLU

MET

3

ILE

SER

ARG

VAL

LEU

ASP

GLU

ASP

4

SER

ARG

LYS

Samples:

sample_1: Fst_toxin .5 mM; potassium phosphate 50 mM; DPC, [U-99% 2H], 100 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 5.0; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

Varian Unity 600 MHz
Bruker Avance 500 MHz

PDB	2KV5
GB	AAF03910 EEI55805 EEN72987 EEU27112 EOE77546
REF	WP_002387930 YP_009074128