BMRB Entry 16757

Title:
Location and properties of metal-binding sites on the human prion protein
Deposition date:
2010-03-03
Original release date:
2010-03-24
Authors:
Jacksn, Graham; Murray, Ian; Hosszu, Laszlo; Gibbs, Nicholas; Waltho, Johnathan; Clarke, Anthony; Collinge, John
Citation:

Citation: Jacksn, Graham; Murray, Ian; Hosszu, Laszlo; Gibbs, Nicholas; Waltho, Johnathan; Clarke, Anthony; Collinge, John. "Location and properties of metal-binding sites on the human prion protein"  Proc. Natl. Acad. Sci. U.S.A. 98, 8531-8535 (2001).
PubMed: 11438695

Assembly members:

Assembly members:
PrP^(91-231), polymer, 145 residues, Formula weight is not available
CU, non-polymer, 63.546 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: purified from the natural source

Data sets:
  • binding_constants
Data typeCount
binding constants1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PrP^(91-231)1
2Cu2

Entities:

Entity 1, PrP^(91-231) 145 residues - Formula weight is not available

91,S

1   SERASPPROGLYGLNGLYGLYGLYTHRHIS
2   SERGLNTRPASNLYSPROSERLYSPROLYS
3   THRASNMETLYSHISMETALAGLYALAALA
4   ALAALAGLYALAVALVALGLYGLYLEUGLY
5   GLYTYRVALLEUGLYSERALAMETSERARG
6   PROILEILEHISPHEGLYSERASPTYRGLU
7   ASPARGTYRTYRARGGLUASNMETHISARG
8   TYRPROASNGLNVALTYRTYRARGPROMET
9   ASPGLUTYRSERASNGLNASNASPPHEVAL
10   HISASNCYSVALASNILETHRILELYSGLN
11   HISTHRVALTHRTHRTHRTHRLYSGLYGLU
12   ASNPHETHRGLUTHRASPVALLYSMETMET
13   GLUARGVALVALGLUGLNMETCYSILETHR
14   GLNTYRGLUARGGLUSERGLNALATYRTYR
15   GLNARGGLYSERSER

Entity 2, Cu - Cu - 63.546 Da.

1   CU

Samples:

sample_1: PrP^(91-231), [U-15N], 500 uM; Mops 5 mM; CuSO4 1000 uM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

FELIX v97, Accelrys Software Inc. - data analysis, processing

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 15676 16743 17714 17756 17780 18426 18550 19268 4402 4434 4641
PDB
DBJ BAA00011 BAG32276 BAG32277 BAG32278 BAG32279
EMBL CAA58442 CAG46836 CAG46869
GB AAA19664 AAA60182 AAA68633 AAB59442 AAB59443
REF NP_000302 NP_001073590 NP_001073591 NP_001073592 NP_898902
SP P04156 P40252
AlphaFold P04156 P40252