BMRB Entry 16744

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PDB ID: 2kuo
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16744
MolProbity Validation Chart

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Title:
Structure and identification of ADP-ribose recognition motifs of APLF and role in the DNA damage response
Deposition date:
2010-02-23
Original release date:
2010-05-18
Authors:
Li, Guang-Yao; McCulloch, Richard; Fenton, Amanda; Cheung, Melissa; Meng, Li; Ikura, Mitsuhiko; Koch, C. Anne
Citation:

Citation: Li, Guang-Yao; McCulloch, Richard; Fenton, Amanda; Cheung, Melissa; Meng, Li; Ikura, Mitsuhiko; Koch, C. Anne. "Structure and identification of ADP-ribose recognition motifs of APLF and role in the DNA damage response."  Proc. Natl. Acad. Sci. U.S.A. 107, 9129-9134 (2010).
PubMed: 20439749

Assembly members:

Assembly members:
APLF_Tandem_ZF, polymer, 91 residues, 10272.400 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX4T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
APLF_Tandem_ZF: GSKATDSVLQGSEGNKVKRT SCMYGANCYRKNPVHFQHFS HPGDSDYGGVQIVGQDETDD RPECPYGPSCYRKNPQHKIE YRHNTLPVRNV

Data sets:
Data typeCount
13C chemical shifts302
15N chemical shifts95
1H chemical shifts608

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2ZINC ION_12
3ZINC ION_22

Entities:

Entity 1, entity_1 91 residues - 10272.400 Da.

The first two residues (GS)are from the experssion vector.The sequence of APLF used in this study is from K360 to V448.

1   GLYSERLYSALATHRASPSERVALLEUGLN
2   GLYSERGLUGLYASNLYSVALLYSARGTHR
3   SERCYSMETTYRGLYALAASNCYSTYRARG
4   LYSASNPROVALHISPHEGLNHISPHESER
5   HISPROGLYASPSERASPTYRGLYGLYVAL
6   GLNILEVALGLYGLNASPGLUTHRASPASP
7   ARGPROGLUCYSPROTYRGLYPROSERCYS
8   TYRARGLYSASNPROGLNHISLYSILEGLU
9   TYRARGHISASNTHRLEUPROVALARGASN
10   VAL

Entity 2, ZINC ION_1 - Zn - 65.409 Da.

1   ZN

Related Database Links:

BMRB 16596
PDB 2KQB 2KQC 2KQD 2KQE 2KUO
DBJ BAF83530
GB AAH41144 AAY24113 ADZ15607 AIC53375 EAW99871
REF NP_775816 XP_003830966 XP_005575759 XP_009235504 XP_009440878
SP Q8IW19
AlphaFold Q8IW19

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