BMRB Entry 16732

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PDB ID: 2kud
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16732
MolProbity Validation Chart

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Title:
NMR structure of the PASTA domain 1 and 2 of Mycobacterium tuberculosis of PknB
Deposition date:
2010-02-17
Original release date:
2010-05-18
Authors:
Barthe, Philippe; Cohen-Gonsaud, Martin; Roumestand, Christian; Mukamolova, Galina
Citation:

Citation: Barthe, Philippe; Mukamolova, Galina; Roumestand, Christian; Cohen-Gonsaud, Martin. "The structure of PknB extracellular PASTA domain from mycobacterium tuberculosis suggests a ligand-dependent kinase activation."  Structure (Cambridge, MA, U. S.) 18, 606-615 (2010).
PubMed: 20462494

Assembly members:

Assembly members:
PknB_PASTA12, polymer, 140 residues, 14203.956 Da.

Natural source:

Natural source:   Common Name: Mycobacterium tuberculosis   Taxonomy ID: 1773   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21-TEV

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PknB_PASTA12: GHMGITRDVQVPDVRGQSSA DAIATLQNRGFKIRTLQKPD STIPPDHVIGTDPAANTSVS AGDEITVNVSTGPEQREIPD VSTLTYAEAVKKLTAAGFGR FKQANSPSTPELVGKVIGTN PPANQTSAITNVVIIIVGSG

Data sets:
Data typeCount
13C chemical shifts392
15N chemical shifts146
1H chemical shifts908

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 140 residues - 14203.956 Da.

1   GLYHISMETGLYILETHRARGASPVALGLN
2   VALPROASPVALARGGLYGLNSERSERALA
3   ASPALAILEALATHRLEUGLNASNARGGLY
4   PHELYSILEARGTHRLEUGLNLYSPROASP
5   SERTHRILEPROPROASPHISVALILEGLY
6   THRASPPROALAALAASNTHRSERVALSER
7   ALAGLYASPGLUILETHRVALASNVALSER
8   THRGLYPROGLUGLNARGGLUILEPROASP
9   VALSERTHRLEUTHRTYRALAGLUALAVAL
10   LYSLYSLEUTHRALAALAGLYPHEGLYARG
11   PHELYSGLNALAASNSERPROSERTHRPRO
12   GLULEUVALGLYLYSVALILEGLYTHRASN
13   PROPROALAASNGLNTHRSERALAILETHR
14   ASNVALVALILEILEILEVALGLYSERGLY

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