Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16727
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Citation: Michalek, Matthias; Wechselberger, Rainer; Soennichsen, Frank; Dingley, Andrew; Wienk, Hans; Simanski, Maren; Herbst, Rosa; Lorenzen, Inken; Gelhaus, Christoph; Grotzinger, Joachim; Leippe, Matthias. "The solution structure of the antimicrobial peptid culberticidin" .
Assembly members:
culbertcidin, polymer, 61 residues, 6001.235 Da.
Natural source: Common Name: Acanthamoeba culbertsoni Taxonomy ID: 43142 Superkingdom: Eukaryota Kingdom: not available Genus/species: Acanthamoeba culbertsoni
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32a
Entity Sequences (FASTA):
culbertcidin: AMGKCSVLKKVACAAAIAGA
VAACGGIDLPCVLAALKAAE
GCASCFCEDHCHGVCKDLHL
C
Data type | Count |
1H chemical shifts | 319 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | culbertcidin | 1 |
Entity 1, culbertcidin 61 residues - 6001.235 Da.
1 | ALA | MET | GLY | LYS | CYS | SER | VAL | LEU | LYS | LYS | ||||
2 | VAL | ALA | CYS | ALA | ALA | ALA | ILE | ALA | GLY | ALA | ||||
3 | VAL | ALA | ALA | CYS | GLY | GLY | ILE | ASP | LEU | PRO | ||||
4 | CYS | VAL | LEU | ALA | ALA | LEU | LYS | ALA | ALA | GLU | ||||
5 | GLY | CYS | ALA | SER | CYS | PHE | CYS | GLU | ASP | HIS | ||||
6 | CYS | HIS | GLY | VAL | CYS | LYS | ASP | LEU | HIS | LEU | ||||
7 | CYS |
sample_1: culbertcidin, [U-100% 15N], 1 mM; culbertcidin 1 mM; H2O 93%; D2O 7%; sodium phosphate 50 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 5.4; pressure: 1 atm; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
DIANA v2.1, Guntert, Braun and Wuthrich - structure solution
NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking, processing