BMRB Entry 16717

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16717

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Title:
Backbone chemical shifts of NI3C DARPin
Deposition date:
2010-02-10
Original release date:
2010-08-12
Authors:
Wetzel, Svava; Ewald, Christina; Jurt, Simon; Zerbe, Oliver; Plueckthun, Andreas
Citation:

Citation: Wetzel, Svava; Ewald, Christina; Settanni, Giovanni; Jurt, Simon; Pluckthun, Andreas; Zerbe, Oliver. "Residue-Resolved Stability of Full-Consensus Ankyrin Repeat Proteins Probed by NMR."  J. Mol. Biol. 402, 241-258 (2010).
PubMed: 20654623

Assembly members:

Assembly members:
NI3C, polymer, 166 residues, 17896.91 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSW_NI3C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NI3C: MRGSHHHHHHGSDLGKKLLE AARAGQDDEVRILMANGADV NAKDKDGYTPLHLAAREGHL EIVEVLLKAGADVNAKDKDG YTPLHLAAREGHLEIVEVLL KAGADVNAKDKDGYTPLHLA AREGHLEIVEVLLKAGADVN AQDKFGKTAFDISIDNGNED LAEILQ

Data sets:
Data typeCount
13C chemical shifts346
15N chemical shifts149
1H chemical shifts149

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NI3C monomer1

Entities:

Entity 1, NI3C monomer 166 residues - 17896.91 Da.

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERASPLEUGLYLYSLYSLEULEUGLU
3   ALAALAARGALAGLYGLNASPASPGLUVAL
4   ARGILELEUMETALAASNGLYALAASPVAL
5   ASNALALYSASPLYSASPGLYTYRTHRPRO
6   LEUHISLEUALAALAARGGLUGLYHISLEU
7   GLUILEVALGLUVALLEULEULYSALAGLY
8   ALAASPVALASNALALYSASPLYSASPGLY
9   TYRTHRPROLEUHISLEUALAALAARGGLU
10   GLYHISLEUGLUILEVALGLUVALLEULEU
11   LYSALAGLYALAASPVALASNALALYSASP
12   LYSASPGLYTYRTHRPROLEUHISLEUALA
13   ALAARGGLUGLYHISLEUGLUILEVALGLU
14   VALLEULEULYSALAGLYALAASPVALASN
15   ALAGLNASPLYSPHEGLYLYSTHRALAPHE
16   ASPILESERILEASPASNGLYASNGLUASP
17   LEUALAGLUILELEUGLN

Related Database Links:

PDB 2QYJ

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks