BMRB Entry 16715

Name: 1H, 13C, and 15N Chemical Shift Assignments for human Vaccinia-Related Kinase-1 Lysozyme
Published: 2011-06-23

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PDB ID: 2lav
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16715
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for human Vaccinia-Related Kinase-1 Lysozyme

Deposition date:

2010-02-10

Original release date:

2011-06-23

Authors:

Shin, Joon; Yoon, Ho Sup

Citation:

Citation: Shin, Joon; Chakraborty, Goutam; Bharatham, Nagakumar; Kang, Congbao; Tochio, Naoya; Koshiba, Seizo; Kigawa, Takanori; Kim, Wanil; Kim, Kyong-Tai; Yoon, Ho Sup. "NMR Solution Structure of Human Vaccinia-related Kinase 1 (VRK1) Reveals the C-terminal Tail Essential for Its Structural Stability and Autocatalytic Activity." J. Biol. Chem. 286, 22131-22138 (2011).
PubMed: 21543316

Assembly members:

Assembly members:
Vaccinia_Related-Kinase_1, polymer, 360 residues, 41286.898 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Vaccinia_Related-Kinase_1: MPRVKAAQAGRQSSAKRHLA EQFAVGEIITDMAKKEWKVG LPIGQGGFGCIYLADMNSSE SVGSDAPCVVKVEPSDNGPL FTELKFYQRAAKPEQIQKWI RTRKLKYLGVPKYWGSGLHD KNGKSYRFMIMDRFGSDLQK IYEANAKRFSRKTVLQLSLR ILDILEYIHEHEYVHGDIKA SNLLLNYKNPDQVYLVDYGL AYRYCPEGVHKEYKEDPKRC HDGTIEFTSIDAHNGVAPSR RGDLEILGYCMIQWLTGHLP WEDNLKDPKYVRDSKIRYRE NIASLMDKCFPEKNKPGEIA KYMETVKLLDYTEKPLYENL RDILLQGLKAIGSKDDGKLD LSVVENGGLKAKTITKKRKK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	1170
15N chemical shifts	337
1H chemical shifts	2038

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Vaccinia_Related-Kinase_1	1

Entities:

Entity 1, Vaccinia_Related-Kinase_1 360 residues - 41286.898 Da.

1	MET	PRO	ARG	VAL	LYS	ALA	ALA	GLN	ALA	GLY
2	ARG	GLN	SER	SER	ALA	LYS	ARG	HIS	LEU	ALA
3	GLU	GLN	PHE	ALA	VAL	GLY	GLU	ILE	ILE	THR
4	ASP	MET	ALA	LYS	LYS	GLU	TRP	LYS	VAL	GLY
5	LEU	PRO	ILE	GLY	GLN	GLY	GLY	PHE	GLY	CYS
6	ILE	TYR	LEU	ALA	ASP	MET	ASN	SER	SER	GLU
7	SER	VAL	GLY	SER	ASP	ALA	PRO	CYS	VAL	VAL
8	LYS	VAL	GLU	PRO	SER	ASP	ASN	GLY	PRO	LEU
9	PHE	THR	GLU	LEU	LYS	PHE	TYR	GLN	ARG	ALA
10	ALA	LYS	PRO	GLU	GLN	ILE	GLN	LYS	TRP	ILE
11	ARG	THR	ARG	LYS	LEU	LYS	TYR	LEU	GLY	VAL
12	PRO	LYS	TYR	TRP	GLY	SER	GLY	LEU	HIS	ASP
13	LYS	ASN	GLY	LYS	SER	TYR	ARG	PHE	MET	ILE
14	MET	ASP	ARG	PHE	GLY	SER	ASP	LEU	GLN	LYS
15	ILE	TYR	GLU	ALA	ASN	ALA	LYS	ARG	PHE	SER
16	ARG	LYS	THR	VAL	LEU	GLN	LEU	SER	LEU	ARG
17	ILE	LEU	ASP	ILE	LEU	GLU	TYR	ILE	HIS	GLU
18	HIS	GLU	TYR	VAL	HIS	GLY	ASP	ILE	LYS	ALA
19	SER	ASN	LEU	LEU	LEU	ASN	TYR	LYS	ASN	PRO
20	ASP	GLN	VAL	TYR	LEU	VAL	ASP	TYR	GLY	LEU
21	ALA	TYR	ARG	TYR	CYS	PRO	GLU	GLY	VAL	HIS
22	LYS	GLU	TYR	LYS	GLU	ASP	PRO	LYS	ARG	CYS
23	HIS	ASP	GLY	THR	ILE	GLU	PHE	THR	SER	ILE
24	ASP	ALA	HIS	ASN	GLY	VAL	ALA	PRO	SER	ARG
25	ARG	GLY	ASP	LEU	GLU	ILE	LEU	GLY	TYR	CYS
26	MET	ILE	GLN	TRP	LEU	THR	GLY	HIS	LEU	PRO
27	TRP	GLU	ASP	ASN	LEU	LYS	ASP	PRO	LYS	TYR
28	VAL	ARG	ASP	SER	LYS	ILE	ARG	TYR	ARG	GLU
29	ASN	ILE	ALA	SER	LEU	MET	ASP	LYS	CYS	PHE
30	PRO	GLU	LYS	ASN	LYS	PRO	GLY	GLU	ILE	ALA
31	LYS	TYR	MET	GLU	THR	VAL	LYS	LEU	LEU	ASP
32	TYR	THR	GLU	LYS	PRO	LEU	TYR	GLU	ASN	LEU
33	ARG	ASP	ILE	LEU	LEU	GLN	GLY	LEU	LYS	ALA
34	ILE	GLY	SER	LYS	ASP	ASP	GLY	LYS	LEU	ASP
35	LEU	SER	VAL	VAL	GLU	ASN	GLY	GLY	LEU	LYS
36	ALA	LYS	THR	ILE	THR	LYS	LYS	ARG	LYS	LYS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1

BMRB	11506 16738
PDB	2KTY 2KUL 2LAV 2RSV 3OP5
DBJ	BAA19108 BAF82799 BAI47070
GB	AAI03762 AAI12076 AAI13511 AAQ02593 AIC55301
REF	NP_001244605 NP_003375 XP_003902302 XP_003928890 XP_004055717
SP	Q99986
AlphaFold	Q99986

BMRB Entry 16715

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	PRO	ARG	VAL	LYS	ALA	ALA	GLN	ALA	GLY
2	ARG	GLN	SER	SER	ALA	LYS	ARG	HIS	LEU	ALA
3	GLU	GLN	PHE	ALA	VAL	GLY	GLU	ILE	ILE	THR
4	ASP	MET	ALA	LYS	LYS	GLU	TRP	LYS	VAL	GLY
5	LEU	PRO	ILE	GLY	GLN	GLY	GLY	PHE	GLY	CYS
6	ILE	TYR	LEU	ALA	ASP	MET	ASN	SER	SER	GLU
7	SER	VAL	GLY	SER	ASP	ALA	PRO	CYS	VAL	VAL
8	LYS	VAL	GLU	PRO	SER	ASP	ASN	GLY	PRO	LEU
9	PHE	THR	GLU	LEU	LYS	PHE	TYR	GLN	ARG	ALA
10	ALA	LYS	PRO	GLU	GLN	ILE	GLN	LYS	TRP	ILE
11	ARG	THR	ARG	LYS	LEU	LYS	TYR	LEU	GLY	VAL
12	PRO	LYS	TYR	TRP	GLY	SER	GLY	LEU	HIS	ASP
13	LYS	ASN	GLY	LYS	SER	TYR	ARG	PHE	MET	ILE
14	MET	ASP	ARG	PHE	GLY	SER	ASP	LEU	GLN	LYS
15	ILE	TYR	GLU	ALA	ASN	ALA	LYS	ARG	PHE	SER
16	ARG	LYS	THR	VAL	LEU	GLN	LEU	SER	LEU	ARG
17	ILE	LEU	ASP	ILE	LEU	GLU	TYR	ILE	HIS	GLU
18	HIS	GLU	TYR	VAL	HIS	GLY	ASP	ILE	LYS	ALA
19	SER	ASN	LEU	LEU	LEU	ASN	TYR	LYS	ASN	PRO
20	ASP	GLN	VAL	TYR	LEU	VAL	ASP	TYR	GLY	LEU
21	ALA	TYR	ARG	TYR	CYS	PRO	GLU	GLY	VAL	HIS
22	LYS	GLU	TYR	LYS	GLU	ASP	PRO	LYS	ARG	CYS
23	HIS	ASP	GLY	THR	ILE	GLU	PHE	THR	SER	ILE
24	ASP	ALA	HIS	ASN	GLY	VAL	ALA	PRO	SER	ARG
25	ARG	GLY	ASP	LEU	GLU	ILE	LEU	GLY	TYR	CYS
26	MET	ILE	GLN	TRP	LEU	THR	GLY	HIS	LEU	PRO
27	TRP	GLU	ASP	ASN	LEU	LYS	ASP	PRO	LYS	TYR
28	VAL	ARG	ASP	SER	LYS	ILE	ARG	TYR	ARG	GLU
29	ASN	ILE	ALA	SER	LEU	MET	ASP	LYS	CYS	PHE
30	PRO	GLU	LYS	ASN	LYS	PRO	GLY	GLU	ILE	ALA
31	LYS	TYR	MET	GLU	THR	VAL	LYS	LEU	LEU	ASP
32	TYR	THR	GLU	LYS	PRO	LEU	TYR	GLU	ASN	LEU
33	ARG	ASP	ILE	LEU	LEU	GLN	GLY	LEU	LYS	ALA
34	ILE	GLY	SER	LYS	ASP	ASP	GLY	LYS	LEU	ASP
35	LEU	SER	VAL	VAL	GLU	ASN	GLY	GLY	LEU	LYS
36	ALA	LYS	THR	ILE	THR	LYS	LYS	ARG	LYS	LYS

1	MET	PRO	ARG	VAL	LYS	ALA	ALA	GLN	ALA	GLY
2	ARG	GLN	SER	SER	ALA	LYS	ARG	HIS	LEU	ALA
3	GLU	GLN	PHE	ALA	VAL	GLY	GLU	ILE	ILE	THR
4	ASP	MET	ALA	LYS	LYS	GLU	TRP	LYS	VAL	GLY
5	LEU	PRO	ILE	GLY	GLN	GLY	GLY	PHE	GLY	CYS
6	ILE	TYR	LEU	ALA	ASP	MET	ASN	SER	SER	GLU
7	SER	VAL	GLY	SER	ASP	ALA	PRO	CYS	VAL	VAL
8	LYS	VAL	GLU	PRO	SER	ASP	ASN	GLY	PRO	LEU
9	PHE	THR	GLU	LEU	LYS	PHE	TYR	GLN	ARG	ALA
10	ALA	LYS	PRO	GLU	GLN	ILE	GLN	LYS	TRP	ILE
11	ARG	THR	ARG	LYS	LEU	LYS	TYR	LEU	GLY	VAL
12	PRO	LYS	TYR	TRP	GLY	SER	GLY	LEU	HIS	ASP
13	LYS	ASN	GLY	LYS	SER	TYR	ARG	PHE	MET	ILE
14	MET	ASP	ARG	PHE	GLY	SER	ASP	LEU	GLN	LYS
15	ILE	TYR	GLU	ALA	ASN	ALA	LYS	ARG	PHE	SER
16	ARG	LYS	THR	VAL	LEU	GLN	LEU	SER	LEU	ARG
17	ILE	LEU	ASP	ILE	LEU	GLU	TYR	ILE	HIS	GLU
18	HIS	GLU	TYR	VAL	HIS	GLY	ASP	ILE	LYS	ALA
19	SER	ASN	LEU	LEU	LEU	ASN	TYR	LYS	ASN	PRO
20	ASP	GLN	VAL	TYR	LEU	VAL	ASP	TYR	GLY	LEU
21	ALA	TYR	ARG	TYR	CYS	PRO	GLU	GLY	VAL	HIS
22	LYS	GLU	TYR	LYS	GLU	ASP	PRO	LYS	ARG	CYS
23	HIS	ASP	GLY	THR	ILE	GLU	PHE	THR	SER	ILE
24	ASP	ALA	HIS	ASN	GLY	VAL	ALA	PRO	SER	ARG
25	ARG	GLY	ASP	LEU	GLU	ILE	LEU	GLY	TYR	CYS
26	MET	ILE	GLN	TRP	LEU	THR	GLY	HIS	LEU	PRO
27	TRP	GLU	ASP	ASN	LEU	LYS	ASP	PRO	LYS	TYR
28	VAL	ARG	ASP	SER	LYS	ILE	ARG	TYR	ARG	GLU
29	ASN	ILE	ALA	SER	LEU	MET	ASP	LYS	CYS	PHE
30	PRO	GLU	LYS	ASN	LYS	PRO	GLY	GLU	ILE	ALA
31	LYS	TYR	MET	GLU	THR	VAL	LYS	LEU	LEU	ASP
32	TYR	THR	GLU	LYS	PRO	LEU	TYR	GLU	ASN	LEU
33	ARG	ASP	ILE	LEU	LEU	GLN	GLY	LEU	LYS	ALA
34	ILE	GLY	SER	LYS	ASP	ASP	GLY	LYS	LEU	ASP
35	LEU	SER	VAL	VAL	GLU	ASN	GLY	GLY	LEU	LYS
36	ALA	LYS	THR	ILE	THR	LYS	LYS	ARG	LYS	LYS

1	MET	PRO	ARG	VAL	LYS	ALA	ALA	GLN	ALA	GLY
2	ARG	GLN	SER	SER	ALA	LYS	ARG	HIS	LEU	ALA
3	GLU	GLN	PHE	ALA	VAL	GLY	GLU	ILE	ILE	THR
4	ASP	MET	ALA	LYS	LYS	GLU	TRP	LYS	VAL	GLY
5	LEU	PRO	ILE	GLY	GLN	GLY	GLY	PHE	GLY	CYS
6	ILE	TYR	LEU	ALA	ASP	MET	ASN	SER	SER	GLU
7	SER	VAL	GLY	SER	ASP	ALA	PRO	CYS	VAL	VAL
8	LYS	VAL	GLU	PRO	SER	ASP	ASN	GLY	PRO	LEU
9	PHE	THR	GLU	LEU	LYS	PHE	TYR	GLN	ARG	ALA
10	ALA	LYS	PRO	GLU	GLN	ILE	GLN	LYS	TRP	ILE
11	ARG	THR	ARG	LYS	LEU	LYS	TYR	LEU	GLY	VAL
12	PRO	LYS	TYR	TRP	GLY	SER	GLY	LEU	HIS	ASP
13	LYS	ASN	GLY	LYS	SER	TYR	ARG	PHE	MET	ILE
14	MET	ASP	ARG	PHE	GLY	SER	ASP	LEU	GLN	LYS
15	ILE	TYR	GLU	ALA	ASN	ALA	LYS	ARG	PHE	SER
16	ARG	LYS	THR	VAL	LEU	GLN	LEU	SER	LEU	ARG
17	ILE	LEU	ASP	ILE	LEU	GLU	TYR	ILE	HIS	GLU
18	HIS	GLU	TYR	VAL	HIS	GLY	ASP	ILE	LYS	ALA
19	SER	ASN	LEU	LEU	LEU	ASN	TYR	LYS	ASN	PRO
20	ASP	GLN	VAL	TYR	LEU	VAL	ASP	TYR	GLY	LEU
21	ALA	TYR	ARG	TYR	CYS	PRO	GLU	GLY	VAL	HIS
22	LYS	GLU	TYR	LYS	GLU	ASP	PRO	LYS	ARG	CYS
23	HIS	ASP	GLY	THR	ILE	GLU	PHE	THR	SER	ILE
24	ASP	ALA	HIS	ASN	GLY	VAL	ALA	PRO	SER	ARG
25	ARG	GLY	ASP	LEU	GLU	ILE	LEU	GLY	TYR	CYS
26	MET	ILE	GLN	TRP	LEU	THR	GLY	HIS	LEU	PRO
27	TRP	GLU	ASP	ASN	LEU	LYS	ASP	PRO	LYS	TYR
28	VAL	ARG	ASP	SER	LYS	ILE	ARG	TYR	ARG	GLU
29	ASN	ILE	ALA	SER	LEU	MET	ASP	LYS	CYS	PHE
30	PRO	GLU	LYS	ASN	LYS	PRO	GLY	GLU	ILE	ALA
31	LYS	TYR	MET	GLU	THR	VAL	LYS	LEU	LEU	ASP
32	TYR	THR	GLU	LYS	PRO	LEU	TYR	GLU	ASN	LEU
33	ARG	ASP	ILE	LEU	LEU	GLN	GLY	LEU	LYS	ALA
34	ILE	GLY	SER	LYS	ASP	ASP	GLY	LYS	LEU	ASP
35	LEU	SER	VAL	VAL	GLU	ASN	GLY	GLY	LEU	LYS
36	ALA	LYS	THR	ILE	THR	LYS	LYS	ARG	LYS	LYS