BMRB Entry 16693

Title:
Solution Structure of a Novel hKv1.1 inhibiting scorpion toxin from Mesibuthus tamulus
Deposition date:
2010-01-26
Original release date:
2011-01-25
Authors:
G., Senthil Kumar; Upadhyay, Sanjeev; M. K., Mathew; P. Sarma, Siddhartha
Citation:

Citation: Kumar, G. Senthil; Upadhyay, Sanjeev; Mathew, M.; Sarma, Siddhartha. "Solution structure of BTK-2, a novel hK(v)1.1 inhibiting scorpion toxin, from the eastern Indian scorpion Mesobuthus tamulus."  Biochim. Biophys. Acta 1814, 459-469 (2011).
PubMed: 21256986

Assembly members:

Assembly members:
BTK2, polymer, 34 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Indian red scorpion   Taxonomy ID: 34647   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Buthus tamulus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BTK2: GSVGCAECPMHCKGKMAKPT CENEVCKCNIGKKD

Data sets:
Data typeCount
13C chemical shifts114
15N chemical shifts31
1H chemical shifts145

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BTK21

Entities:

Entity 1, BTK2 34 residues - Formula weight is not available

GS at the N-terminus is due to cloning exigencies

1   GLYSERVALGLYCYSALAGLUCYSPROMET
2   HISCYSLYSGLYLYSMETALALYSPROTHR
3   CYSGLUASNGLUVALCYSLYSCYSASNILE
4   GLYLYSLYSASP

Samples:

15N_13C_BTK2: BTK2, [U-99% 13C; U-99% 15N], 1 – 2 mM

sample_2: BTK21 – 2 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15N_13C_BTK2isotropicsample_conditions_1
2D 1H-13C HSQC15N_13C_BTK2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
3D CBCA(CO)NH15N_13C_BTK2isotropicsample_conditions_1
3D C(CO)NH15N_13C_BTK2isotropicsample_conditions_1
3D HNCO15N_13C_BTK2isotropicsample_conditions_1
3D HNCACB15N_13C_BTK2isotropicsample_conditions_1
3D H(CCO)NH15N_13C_BTK2isotropicsample_conditions_1
3D HCCH-COSY15N_13C_BTK2isotropicsample_conditions_1
3D HNHA15N_13C_BTK2isotropicsample_conditions_1
3D 1H-15N NOESY15N_13C_BTK2isotropicsample_conditions_1
3D 1H-13C NOESY15N_13C_BTK2isotropicsample_conditions_1
3D HACAHBCOSY15N_13C_BTK2isotropicsample_conditions_1

Software:

X-PLOR NIH, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Koradi, Billeter and Wuthrich, Kraulis, Schwieters, Kuszewski, Tjandra and Clore - data analysis, data analysis, structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 500 MHz

Related Database Links:

PDB
SP P60209
AlphaFold P60209

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks