BMRB Entry 16661

Title:
Pf ACP with Transit Peptide
Deposition date:
2010-01-04
Original release date:
2010-01-12
Authors:
Gallagher, John; Prigge, Sean
Citation:

Citation: Gallagher, John; Prigge, Sean. "Plasmodium falciparum acyl carrier protein crystal structures in disulfide-linked and reduced states and their prevalence during blood stage growth."  Proteins 78, 575-588 (2010).
PubMed: 19768685

Assembly members:

Assembly members:
TP-ACP, polymer, 121 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: malaria parasite P. falciparum   Taxonomy ID: 5833   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Plasmodium falciparum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSPr026

Data sets:
Data typeCount
13C chemical shifts238
15N chemical shifts119
1H chemical shifts119

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TP-ACP1

Entities:

Entity 1, TP-ACP 121 residues - Formula weight is not available

Represents trafficking intermediate with signal peptide removed, containing residues 18-137 of Genbank AAC71866.

1   PHELYSASNTHRGLNLYSASPGLYVALSER
2   LEUGLNILELEULYSLYSLYSARGSERASN
3   GLNVALASNPHELEUASNARGLYSASNASP
4   TYRASNLEUILELYSASNLYSASNPROSER
5   SERSERLEULYSSERTHRPHEASPASPILE
6   LYSLYSILEILESERLYSGLNLEUSERVAL
7   GLUGLUASPLYSILEGLNMETASNSERASN
8   PHETHRLYSASPLEUGLYALAASPSERLEU
9   ASPLEUVALGLULEUILEMETALALEUGLU
10   GLULYSPHEASNVALTHRILESERASPGLN
11   ASPALALEULYSILEASNTHRVALGLNASP
12   ALAILEASPTYRILEGLULYSASNASNLYS
13   GLN

Samples:

sample_1: TP-ACP, [U-13C; U-15N], 940 uM; D3NaAcetate, [U-2H], 30 mM; CaCl2 20 mM; DTT 5 mM; sodium azide 1 mM; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking

MONTE v2.02, Hitchens, T. K. Lukin, J. A. Zhan, Y. McCallum, S. A. Rule, G. S. - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

GB AAC71866 AAC63959 AAC71866 ETW20884 ETW27965 ETW39040
BMRB 16506 16529 16530 16531 16532 16533
PDB
EMBL CDO62314
REF XP_001349595 XP_012760964

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks