BMRB Entry 16661

Name: Pf ACP with Transit Peptide
Published: 2010-01-12

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16661

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Pf ACP with Transit Peptide

Deposition date:

2010-01-04

Original release date:

2010-01-12

Authors:

Gallagher, John; Prigge, Sean

Citation:

Citation: Gallagher, John; Prigge, Sean. "Plasmodium falciparum acyl carrier protein crystal structures in disulfide-linked and reduced states and their prevalence during blood stage growth." Proteins 78, 575-588 (2010).
PubMed: 19768685

Assembly members:

Assembly members:
TP-ACP, polymer, 121 residues, Formula weight is not available

Natural source:

Natural source: Common Name: malaria parasite P. falciparum Taxonomy ID: 5833 Superkingdom: Eukaryota Kingdom: not available Genus/species: Plasmodium falciparum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSPr026

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TP-ACP: FKNTQKDGVSLQILKKKRSN QVNFLNRKNDYNLIKNKNPS SSLKSTFDDIKKIISKQLSV EEDKIQMNSNFTKDLGADSL DLVELIMALEEKFNVTISDQ DALKINTVQDAIDYIEKNNK Q

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	238
15N chemical shifts	119
1H chemical shifts	119

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TP-ACP	1

Entities:

Entity 1, TP-ACP 121 residues - Formula weight is not available

Represents trafficking intermediate with signal peptide removed, containing residues 18-137 of Genbank AAC71866.

1

PHE

LYS

ASN

THR

GLN

LYS

ASP

GLY

VAL

SER

2

LEU

GLN

ILE

LEU

LYS

ARG

SER

ASN

3

GLN

VAL

ASN

PHE

LEU

ASN

ARG

LYS

ASN

ASP

4

TYR

ASN

LEU

ILE

LYS

ASN

LYS

ASN

PRO

SER

5

SER

LEU

LYS

SER

THR

PHE

ASP

ILE

6

LYS

ILE

SER

LYS

GLN

LEU

SER

VAL

7

GLU

ASP

LYS

ILE

GLN

MET

ASN

SER

ASN

8

PHE

THR

LYS

ASP

LEU

GLY

ALA

ASP

SER

LEU

9

ASP

LEU

VAL

GLU

LEU

ILE

MET

ALA

LEU

GLU

10

GLU

LYS

PHE

ASN

VAL

THR

ILE

SER

ASP

GLN

11

ASP

ALA

LEU

LYS

ILE

ASN

THR

VAL

GLN

ASP

12

ALA

ILE

ASP

TYR

ILE

GLU

LYS

ASN

LYS

13

GLN

Samples:

sample_1: TP-ACP, [U-13C; U-15N], 940 uM; D3NaAcetate, [U-2H], 30 mM; CaCl2 20 mM; DTT 5 mM; sodium azide 1 mM; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking

MONTE v2.02, Hitchens, T. K. Lukin, J. A. Zhan, Y. McCallum, S. A. Rule, G. S. - chemical shift assignment

NMR spectrometers:

Varian INOVA 500 MHz
Varian INOVA 600 MHz

GB	AAC71866 AAC63959 AAC71866 ETW20884 ETW27965 ETW39040
BMRB	16506 16529 16530 16531 16532 16533
PDB	2FQ0 2FQ2 3GZL 3GZM
EMBL	CDO62314
REF	XP_001349595 XP_012760964