BMRB Entry 16646

Name: Solution structure of the RBD1,2 domains from human nucleolin
Published: 2010-05-13

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PDB ID: 2krr
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16646
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the RBD1,2 domains from human nucleolin

Deposition date:

2009-12-22

Original release date:

2010-05-13

Authors:

Arumugam, Sengodagounder; Miller, Clarke; Maliekal, James; Bates, Paula; Trent, John; Lane, Andrew

Citation:

Citation: Arumugam, Sengodagounder; Miller, M. Clarke; Maliekal, James; Bates, Paula; Trent, John; Lane, Andrew. "Solution structure of the RBD1,2 domains from human nucleolin." J. Biomol. NMR 47, 79-83 (2010).
PubMed: 20376532

Assembly members:

Assembly members:
RBD1,2, polymer, 180 residues, 20175.620 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RBD1,2: GTEPTTAFNLFVGNLNFNKS APELKTGISDVFAKNDLAVV DVRIGMTRKFGYVDFESAED LEKALELTGLKVFGNEIKLE KPKGKDSKKERDARTLLAKN LPYKVTQDELKEVFEDAAEI RLVSKDGKSKGIAYIEFKTE ADAEKTFEEKQGTEIDGRSI SLYYTGEPKGEGLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	725
15N chemical shifts	180
1H chemical shifts	1091

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity	1

Entities:

Entity 1, entity 180 residues - 20175.620 Da.

1	GLY	THR	GLU	PRO	THR	THR	ALA	PHE	ASN	LEU
2	PHE	VAL	GLY	ASN	LEU	ASN	PHE	ASN	LYS	SER
3	ALA	PRO	GLU	LEU	LYS	THR	GLY	ILE	SER	ASP
4	VAL	PHE	ALA	LYS	ASN	ASP	LEU	ALA	VAL	VAL
5	ASP	VAL	ARG	ILE	GLY	MET	THR	ARG	LYS	PHE
6	GLY	TYR	VAL	ASP	PHE	GLU	SER	ALA	GLU	ASP
7	LEU	GLU	LYS	ALA	LEU	GLU	LEU	THR	GLY	LEU
8	LYS	VAL	PHE	GLY	ASN	GLU	ILE	LYS	LEU	GLU
9	LYS	PRO	LYS	GLY	LYS	ASP	SER	LYS	LYS	GLU
10	ARG	ASP	ALA	ARG	THR	LEU	LEU	ALA	LYS	ASN
11	LEU	PRO	TYR	LYS	VAL	THR	GLN	ASP	GLU	LEU
12	LYS	GLU	VAL	PHE	GLU	ASP	ALA	ALA	GLU	ILE
13	ARG	LEU	VAL	SER	LYS	ASP	GLY	LYS	SER	LYS
14	GLY	ILE	ALA	TYR	ILE	GLU	PHE	LYS	THR	GLU
15	ALA	ASP	ALA	GLU	LYS	THR	PHE	GLU	GLU	LYS
16	GLN	GLY	THR	GLU	ILE	ASP	GLY	ARG	SER	ILE
17	SER	LEU	TYR	TYR	THR	GLY	GLU	PRO	LYS	GLY
18	GLU	GLY	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1

BMRB Entry 16646

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	THR	GLU	PRO	THR	THR	ALA	PHE	ASN	LEU
2	PHE	VAL	GLY	ASN	LEU	ASN	PHE	ASN	LYS	SER
3	ALA	PRO	GLU	LEU	LYS	THR	GLY	ILE	SER	ASP
4	VAL	PHE	ALA	LYS	ASN	ASP	LEU	ALA	VAL	VAL
5	ASP	VAL	ARG	ILE	GLY	MET	THR	ARG	LYS	PHE
6	GLY	TYR	VAL	ASP	PHE	GLU	SER	ALA	GLU	ASP
7	LEU	GLU	LYS	ALA	LEU	GLU	LEU	THR	GLY	LEU
8	LYS	VAL	PHE	GLY	ASN	GLU	ILE	LYS	LEU	GLU
9	LYS	PRO	LYS	GLY	LYS	ASP	SER	LYS	LYS	GLU
10	ARG	ASP	ALA	ARG	THR	LEU	LEU	ALA	LYS	ASN
11	LEU	PRO	TYR	LYS	VAL	THR	GLN	ASP	GLU	LEU
12	LYS	GLU	VAL	PHE	GLU	ASP	ALA	ALA	GLU	ILE
13	ARG	LEU	VAL	SER	LYS	ASP	GLY	LYS	SER	LYS
14	GLY	ILE	ALA	TYR	ILE	GLU	PHE	LYS	THR	GLU
15	ALA	ASP	ALA	GLU	LYS	THR	PHE	GLU	GLU	LYS
16	GLN	GLY	THR	GLU	ILE	ASP	GLY	ARG	SER	ILE
17	SER	LEU	TYR	TYR	THR	GLY	GLU	PRO	LYS	GLY
18	GLU	GLY	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	GLY	THR	GLU	PRO	THR	THR	ALA	PHE	ASN	LEU
2	PHE	VAL	GLY	ASN	LEU	ASN	PHE	ASN	LYS	SER
3	ALA	PRO	GLU	LEU	LYS	THR	GLY	ILE	SER	ASP
4	VAL	PHE	ALA	LYS	ASN	ASP	LEU	ALA	VAL	VAL
5	ASP	VAL	ARG	ILE	GLY	MET	THR	ARG	LYS	PHE
6	GLY	TYR	VAL	ASP	PHE	GLU	SER	ALA	GLU	ASP
7	LEU	GLU	LYS	ALA	LEU	GLU	LEU	THR	GLY	LEU
8	LYS	VAL	PHE	GLY	ASN	GLU	ILE	LYS	LEU	GLU
9	LYS	PRO	LYS	GLY	LYS	ASP	SER	LYS	LYS	GLU
10	ARG	ASP	ALA	ARG	THR	LEU	LEU	ALA	LYS	ASN
11	LEU	PRO	TYR	LYS	VAL	THR	GLN	ASP	GLU	LEU
12	LYS	GLU	VAL	PHE	GLU	ASP	ALA	ALA	GLU	ILE
13	ARG	LEU	VAL	SER	LYS	ASP	GLY	LYS	SER	LYS
14	GLY	ILE	ALA	TYR	ILE	GLU	PHE	LYS	THR	GLU
15	ALA	ASP	ALA	GLU	LYS	THR	PHE	GLU	GLU	LYS
16	GLN	GLY	THR	GLU	ILE	ASP	GLY	ARG	SER	ILE
17	SER	LEU	TYR	TYR	THR	GLY	GLU	PRO	LYS	GLY
18	GLU	GLY	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	GLY	THR	GLU	PRO	THR	THR	ALA	PHE	ASN	LEU
2	PHE	VAL	GLY	ASN	LEU	ASN	PHE	ASN	LYS	SER
3	ALA	PRO	GLU	LEU	LYS	THR	GLY	ILE	SER	ASP
4	VAL	PHE	ALA	LYS	ASN	ASP	LEU	ALA	VAL	VAL
5	ASP	VAL	ARG	ILE	GLY	MET	THR	ARG	LYS	PHE
6	GLY	TYR	VAL	ASP	PHE	GLU	SER	ALA	GLU	ASP
7	LEU	GLU	LYS	ALA	LEU	GLU	LEU	THR	GLY	LEU
8	LYS	VAL	PHE	GLY	ASN	GLU	ILE	LYS	LEU	GLU
9	LYS	PRO	LYS	GLY	LYS	ASP	SER	LYS	LYS	GLU
10	ARG	ASP	ALA	ARG	THR	LEU	LEU	ALA	LYS	ASN
11	LEU	PRO	TYR	LYS	VAL	THR	GLN	ASP	GLU	LEU
12	LYS	GLU	VAL	PHE	GLU	ASP	ALA	ALA	GLU	ILE
13	ARG	LEU	VAL	SER	LYS	ASP	GLY	LYS	SER	LYS
14	GLY	ILE	ALA	TYR	ILE	GLU	PHE	LYS	THR	GLU
15	ALA	ASP	ALA	GLU	LYS	THR	PHE	GLU	GLU	LYS
16	GLN	GLY	THR	GLU	ILE	ASP	GLY	ARG	SER	ILE
17	SER	LEU	TYR	TYR	THR	GLY	GLU	PRO	LYS	GLY
18	GLU	GLY	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS