BMRB Entry 16608

Click here to enlarge.

PDB ID: 2kqp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16608
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
NMR Structure of Proinsulin
Deposition date:
2009-11-13
Original release date:
2010-03-15
Authors:
Yang, Yanwu; Hua, Qing-hua; Mackin, Robert; Weiss, Michael
Citation:

Citation: Yang, Yanwu; Hua, Qing-Xin; Liu, Jin; Shimizu, Eri; Choquette, Meredith; Mackin, Robert; Weiss, Michael. "Solution Structure of Proinsulin: CONNECTING DOMAIN FLEXIBILITY AND PROHORMONE PROCESSING."  J. Biol. Chem. 285, 7847-7851 (2010).
PubMed: 20106974

Assembly members:

Assembly members:
Proinsulin, polymer, 86 residues, 9379.647 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-9b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Proinsulin: FVNQHLCGSDLVEALYLVCG ERGFFYTKPTRREAEDLQVG QVELGGGPGAGSLQPLALEG SLQKRGIVEQCCTSICSLYQ LENYCN

Data sets:
Data typeCount
13C chemical shifts271
15N chemical shifts83
1H chemical shifts568

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Proinsulin1

Entities:

Entity 1, Proinsulin 86 residues - 9379.647 Da.

1   PHEVALASNGLNHISLEUCYSGLYSERASP
2   LEUVALGLUALALEUTYRLEUVALCYSGLY
3   GLUARGGLYPHEPHETYRTHRLYSPROTHR
4   ARGARGGLUALAGLUASPLEUGLNVALGLY
5   GLNVALGLULEUGLYGLYGLYPROGLYALA
6   GLYSERLEUGLNPROLEUALALEUGLUGLY
7   SERLEUGLNLYSARGGLYILEVALGLUGLN
8   CYSCYSTHRSERILECYSSERLEUTYRGLN
9   LEUGLUASNTYRCYSASN

Related Database Links:

PDB 2KQP

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks