BMRB Entry 16595
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16595
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Citation: Qiang, Wei; Bodner, Michele; Weliky, David. "Solid-state NMR Spectroscopy of human immunodeficiency virus fusion peptides associated with host-cell-like membranes: 2D correlation spectra and distance measurements support a fully extended conformation and models for specific antiparallel strand registries" J. Am. Chem. Soc. 130, 5459-5471 (2008).
PubMed: 18370385
Assembly members:
HIV_Fusion_Peptide, polymer, 31 residues, Formula weight is not available
Natural source: Common Name: HIV Taxonomy ID: 12721 Superkingdom: virus Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
HIV_Fusion_Peptide: AVGIGALFLGFLGAAGSAMG
ARSCKKKKKKW
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 70 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HIV Fusion Peptide | 1 |
Entities:
Entity 1, HIV Fusion Peptide 31 residues - Formula weight is not available
| 1 | ALA | VAL | GLY | ILE | GLY | ALA | LEU | PHE | LEU | GLY | ||||
| 2 | PHE | LEU | GLY | ALA | ALA | GLY | SER | ALA | MET | GLY | ||||
| 3 | ALA | ARG | SER | CYS | LYS | LYS | LYS | LYS | LYS | LYS | ||||
| 4 | TRP |
Samples:
sample_1: HIV Fusion Peptide, [U-99% 13C; U-99% 15N], 20 mM; Membrane 750 mM; HEPES 5 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 3 mM; pH: 7.0; pressure: 1 atm; temperature: 223 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 13C-13C spin diffusion | sample_1 | solid | sample_conditions_1 |
Software:
NMRPipe, Cornilescu, Delaglio and Bax, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian Infinity Plus 400 MHz