BMRB Entry 16518

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16518

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Title:
Backbone resonance assignments for oxidised Trypanosoma cruzi glutathione peroxidase
Deposition date:
2009-09-24
Original release date:
2009-11-20
Authors:
Driscoll, Paul; Harris, Richard; Patel, Shreenal; Djordjevic, Snezana
Citation:

Citation: Patel, Shreenal; Hussain, Syeed; Harris, Richard; Sardiwal, Sunita; Kelly, John; Wilkinson, Shane; Driscoll, Paul; Djordjevic, Snezana. "Structural insights into the catalytic mechanism of Trypanosoma cruzi GPXI (glutathione peroxidase-like enzyme I)."  Biochem. J. 425, 513-522 (2010).
PubMed: 19886864

Assembly members:

Assembly members:
TcGPXI, polymer, 168 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Trypanosoma cruzi   Taxonomy ID: 5693   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma cruzi

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Rosetta (DE3)PLysS

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TcGPXI: GAKSIYEFQVNAADGKPYDL SQHKGHPLLIYNVASRCGYT KGGYETATTLYNKYKGQGFT VLAFPCNQFAGQEPGTALEV KEFACTRFKADFPIMAKIDV NGSGAKAHPLYEFMKATIPG LFGTKAIKWNFTSFLIDRHG VPVERFSPGASVEDIEKKLL PLLGGARI

Data sets:
Data typeCount
13C chemical shifts473
15N chemical shifts149
1H chemical shifts323

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TcGPXI1

Entities:

Entity 1, TcGPXI 168 residues - Formula weight is not available

1   GLYALALYSSERILETYRGLUPHEGLNVAL
2   ASNALAALAASPGLYLYSPROTYRASPLEU
3   SERGLNHISLYSGLYHISPROLEULEUILE
4   TYRASNVALALASERARGCYSGLYTYRTHR
5   LYSGLYGLYTYRGLUTHRALATHRTHRLEU
6   TYRASNLYSTYRLYSGLYGLNGLYPHETHR
7   VALLEUALAPHEPROCYSASNGLNPHEALA
8   GLYGLNGLUPROGLYTHRALALEUGLUVAL
9   LYSGLUPHEALACYSTHRARGPHELYSALA
10   ASPPHEPROILEMETALALYSILEASPVAL
11   ASNGLYSERGLYALALYSALAHISPROLEU
12   TYRGLUPHEMETLYSALATHRILEPROGLY
13   LEUPHEGLYTHRLYSALAILELYSTRPASN
14   PHETHRSERPHELEUILEASPARGHISGLY
15   VALPROVALGLUARGPHESERPROGLYALA
16   SERVALGLUASPILEGLULYSLYSLEULEU
17   PROLEULEUGLYGLYALAARGILE

Samples:

sample_1: TcGPXI, [U-99% 13C; U-99% 15N], 0.8 mM; phosphate 50 mM; sodium chloride 300 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.350 M; pH: 6.5; pressure: 1.0 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HA(CA)NHsample_1isotropicsample_conditions_1
3D HA(CO)NHsample_1isotropicsample_conditions_1

Software:

ANSIG, Kraulis - chemical shift assignment

NMR spectrometers:

  • Varian UnityPlus 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
EMBL CAC85914
GB EAN90957 ESS69943
REF XP_812808

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks