BMRB Entry 16502
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16502
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Citation: Pineda-Lucena, Antonio; Jimenez, M. Angeles; Nieto, Jose; Santoro, Jorge; Rico, Manuel; Gimenez-Gallego, Guillermo. "Solution structure of acidic fibroblast growth factor bound to 1,3, 6-naphthalenetrisulfonate: a minimal model for the anti-tumoral action of suramins and suradistas." J. Mol. Biol. 281, 899-915 (1998).
PubMed: 9719643
Assembly members:
aFGF, polymer, 132 residues, Formula weight is not available
NTS, non-polymer, 365.336 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMG47
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 790 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | acidic fibroblast growth factor | 1 |
| 2 | 1,3,6-naphthalenetrisulfonate | 2 |
Entities:
Entity 1, acidic fibroblast growth factor 132 residues - Formula weight is not available
Protein construct lacks the 22 N-terminal residues.
| 1 | LYS | LYS | PRO | LYS | LEU | LEU | TYR | CYS | SER | ASN | ||||
| 2 | GLY | GLY | HIS | PHE | LEU | ARG | ILE | LEU | PRO | ASP | ||||
| 3 | GLY | THR | VAL | ASP | GLY | THR | ARG | ASP | ARG | SER | ||||
| 4 | ASP | GLN | HIS | ILE | GLN | LEU | GLN | LEU | SER | ALA | ||||
| 5 | GLU | SER | VAL | GLY | GLU | VAL | TYR | ILE | LYS | SER | ||||
| 6 | THR | GLU | THR | GLY | GLN | TYR | LEU | ALA | MET | ASP | ||||
| 7 | THR | ASP | GLY | LEU | LEU | TYR | GLY | SER | GLN | THR | ||||
| 8 | PRO | ASN | GLU | GLU | CYS | LEU | PHE | LEU | GLU | ARG | ||||
| 9 | LEU | GLU | GLU | ASN | HIS | TYR | ASN | THR | TYR | ILE | ||||
| 10 | SER | LYS | LYS | HIS | ALA | GLU | LYS | ASN | TRP | PHE | ||||
| 11 | VAL | GLY | LEU | LYS | LYS | ASN | GLY | SER | CYS | LYS | ||||
| 12 | ARG | GLY | PRO | ARG | THR | HIS | TYR | GLY | GLN | LYS | ||||
| 13 | ALA | ILE | LEU | PHE | LEU | PRO | LEU | PRO | VAL | SER | ||||
| 14 | SER | ASP |
Entity 2, 1,3,6-naphthalenetrisulfonate - C10 H5 O9 S3 - 365.336 Da.
| 1 | NTS |