BMRB Entry 16487

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16487

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Title:
Backbone 1H, 15N and 13C chemical shift assignments for HCV-1b Core+1/S polypeptide.
Deposition date:
2009-09-09
Original release date:
2010-01-28
Authors:
Boumlic, Anissa; Nomine, Yves; Kieffer, Bruno; Trave, Gilles
Citation:

Citation: Boumlic, Anissa; Nomine, Yves; Charbonnier, Sebastian; Dalagiorgou, Georgia; Vassilaki, Niki; Kieffer, Bruno; Trave, Gilles; Mavromara, Penelope; Orfanoudakis, Georges. "Prevalence of intrinsic disorder in the hepatitis C virus ARFP/Core+1/S protein."  FEBS J. 277, 774-789 (2010).
PubMed: 20067524

Assembly members:

Assembly members:
HCV-1b_Core+1/S, polymer, 60 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Hepatitis C virus   Taxonomy ID: 11103   Superkingdom: Viruses   Kingdom: not available   Genus/species: Hepacivirus Hepatitis C virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-20b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HCV-1b_Core+1/S: GAMAMRAWGGQDGSCHPVAP GLVGAPMTPGVGRVIWVRSS IPSRAASPTSWGTFRSSAPP

Data sets:
Data typeCount
13C chemical shifts157
15N chemical shifts48
1H chemical shifts48

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit 11

Entities:

Entity 1, unit 1 60 residues - Formula weight is not available

Residues -1 and 0 result from the cloning strategy.

1   GLYALAMETALAMETARGALATRPGLYGLY
2   GLNASPGLYSERCYSHISPROVALALAPRO
3   GLYLEUVALGLYALAPROMETTHRPROGLY
4   VALGLYARGVALILETRPVALARGSERSER
5   ILEPROSERARGALAALASERPROTHRSER
6   TRPGLYTHRPHEARGSERSERALAPROPRO

Samples:

sample_1: HCV-1b Core+1/S, [U-100% 13C; U-100% 15N], 400 uM; sodium phosphate 20 mM; sodium chloride 400 mM; DTT 2 mM; Arginine 50 mM; Glutamic acid 50 mM; D2O 7%; H2O 93%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker DRX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks