BMRB Entry 16441

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PDB ID: 2kmx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16441
MolProbity Validation Chart

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Title:
Solution structure of the nucleotide binding domain of the human Menkes protein in the ATP-bound form
Deposition date:
2009-08-05
Original release date:
2009-11-19
Authors:
Banci, Lucia; Bertini, Ivano; Cantini, Francesca; Inagaki, Sayaka; Migliardi, Manuele; Rosato, Antonio
Citation:

Citation: Banci, Lucia; Bertini, Ivano; Cantini, Francesca; Inagaki, Sayaka; Migliardi, Manuele; Rosato, Antonio. "The Binding Mode of ATP Revealed by the Solution Structure of the N-domain of Human ATP7A."  J. Biol. Chem. 285, 2537-2544 (2010).
PubMed: 19917612

Assembly members:

Assembly members:
N-MNK, polymer, 185 residues, 19803.387 Da.
ATP, non-polymer, 507.181 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETG-30A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N-MNK: SFTMHGTPVVNQVKVLTESN RISHHKILAIVGTAESNSEH PLGTAITKYCKQELDTETLG TCIDFQVVPGCGISCKVTNI EGLLHKNNWNIEDNNIKNAS LVQIDASNEQSSTSSSMIID AQISNALNAQQYKVLIGNRE WMIRNGLVINNDVNDFMTEH ERKGRTAVLVAVDDELCGLI AIADT

Data sets:
Data typeCount
13C chemical shifts675
15N chemical shifts193
1H chemical shifts1186

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1N-MNK1
2ATP2

Entities:

Entity 1, N-MNK 185 residues - 19803.387 Da.

After cleavage of the tag, four amino acids (SFTM) from the restriction site are left at the N-terminus of the protein.

1   SERPHETHRMETHISGLYTHRPROVALVAL
2   ASNGLNVALLYSVALLEUTHRGLUSERASN
3   ARGILESERHISHISLYSILELEUALAILE
4   VALGLYTHRALAGLUSERASNSERGLUHIS
5   PROLEUGLYTHRALAILETHRLYSTYRCYS
6   LYSGLNGLULEUASPTHRGLUTHRLEUGLY
7   THRCYSILEASPPHEGLNVALVALPROGLY
8   CYSGLYILESERCYSLYSVALTHRASNILE
9   GLUGLYLEULEUHISLYSASNASNTRPASN
10   ILEGLUASPASNASNILELYSASNALASER
11   LEUVALGLNILEASPALASERASNGLUGLN
12   SERSERTHRSERSERSERMETILEILEASP
13   ALAGLNILESERASNALALEUASNALAGLN
14   GLNTYRLYSVALLEUILEGLYASNARGGLU
15   TRPMETILEARGASNGLYLEUVALILEASN
16   ASNASPVALASNASPPHEMETTHRGLUHIS
17   GLUARGLYSGLYARGTHRALAVALLEUVAL
18   ALAVALASPASPGLULEUCYSGLYLEUILE
19   ALAILEALAASPTHR

Entity 2, ATP - C10 H16 N5 O13 P3 - 507.181 Da.

1   ATP

Related Database Links:

PIR S36149
SWS NP_000043.3
BMRB 16440
PDB 2KMV 2KMX
DBJ BAG61422
EMBL CAB08162 CAB94714
GB AAA35580 AAA96010 AAI56438 EAW98605 EAW98606
REF NP_000043 NP_001269153 XP_002831882 XP_003824455 XP_004064479
SP Q04656
AlphaFold Q04656

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