BMRB Entry 16428

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PDB ID: 2kmg
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16428
MolProbity Validation Chart

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Title:
The structure of the KlcA and ArdB proteins show a novel fold and antirestriction activity against Type I DNA restriction systems in vivo but not in vitro.
Deposition date:
2009-07-28
Original release date:
2010-02-02
Authors:
Serfiotis-Mitsa, Dimitra; Herbert, Andrew; Roberts, Gareth; Soares, Dinesh; White, John; Blakely, Garry; Uhrin, Dusan; Dryden, David
Citation:

Citation: Serfiotis-Mitsa, Dimitra; Herbert, Andrew; Roberts, Gareth; Soares, Dinesh; White, John; Blakely, Garry; Uhrin, Dusan; Dryden, David. "The structure of the KlcA and ArdB proteins reveals a novel fold and antirestriction activity against Type I DNA restriction systems in vivo but not in vitro."  Nucleic Acids Res. 38, 1723-1737 (2010).
PubMed: 20007596

Assembly members:

Assembly members:
KlcA and ArdB proteins, polymer, 142 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: B. pertussis   Taxonomy ID: 520   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bordetella pertussis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KlcA and ArdB proteins: MNTEEQPVTASLVAEAQRLD FLPTYFGPRLMMRGEALVYA WMRRLCERYNGAYWHYYALS DGGFYMAPDLAGRLEIEVNG NGFRGELSADAAGIVATLFA LGQLAAEIADTDAADALIDR YHFLRGFAAGHPEAAAIYRA ID

Data sets:
Data typeCount
13C chemical shifts559
15N chemical shifts149
1H chemical shifts897
residual dipolar couplings110

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1KlcA and ArdB proteins1

Entities:

Entity 1, KlcA and ArdB proteins 142 residues - Formula weight is not available

1   METASNTHRGLUGLUGLNPROVALTHRALA
2   SERLEUVALALAGLUALAGLNARGLEUASP
3   PHELEUPROTHRTYRPHEGLYPROARGLEU
4   METMETARGGLYGLUALALEUVALTYRALA
5   TRPMETARGARGLEUCYSGLUARGTYRASN
6   GLYALATYRTRPHISTYRTYRALALEUSER
7   ASPGLYGLYPHETYRMETALAPROASPLEU
8   ALAGLYARGLEUGLUILEGLUVALASNGLY
9   ASNGLYPHEARGGLYGLULEUSERALAASP
10   ALAALAGLYILEVALALATHRLEUPHEALA
11   LEUGLYGLNLEUALAALAGLUILEALAASP
12   THRASPALAALAASPALALEUILEASPARG
13   TYRHISPHELEUARGGLYPHEALAALAGLY
14   HISPROGLUALAALAALAILETYRARGALA
15   ILEASP

Related Database Links:

EMBL BAF33451.1
UNP Q08L07
PDB 2KMG
DBJ BAF33451
REF WP_011666376 YP_787932
AlphaFold Q08L07

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