BMRB Entry 16422

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16422

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution Structure and Dynamics of the N-terminal Cystosolic Domain of Rhomboid Intramembrane Protease from Pseudomonas aeruginosa: Insights into a Functional Role in Intramembrane Proteolysis
Deposition date:
2009-07-23
Original release date:
2010-03-08
Authors:
Del Rio, Armando; Dutta, Kaushik; Chavez, Jose; Ubarretxena-Belandia, Iban; Ghose, Ranajeet
Citation:

Citation: Del Rio, Armando; Dutta, Kaushik; Chavez, Jose; Ubarretxena-Belandia, Iban; Ghose, Ranajeet. "Solution Structure and Dynamics of the N-terminal Cystosolic Domain of Rhomboid Intramembrane Protease from Pseudomonas aeruginosa: Insights into a Functional Role in Intramembrane Proteolysis"  J. Mol. Biol. 1, 109-122 (2007).
PubMed: 17059825

Assembly members:

Assembly members:
NRho, polymer, 70 residues, 407.568 Da.
C16PN, non-polymer, 407.568 Da.

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NRho: MSAVQVLKFPLSVDLAGFVG LLRRLNVPHRVSEESGQQVL WVPDERLAEQVRELYRRYPE GDPQATLEAA

Data sets:
  • binding_constants
Data typeCount
binding constants8

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NRho1
2C16PN2

Entities:

Entity 1, NRho 70 residues - 407.568 Da.

1   METSERALAVALGLNVALLEULYSPHEPRO
2   LEUSERVALASPLEUALAGLYPHEVALGLY
3   LEULEUARGARGLEUASNVALPROHISARG
4   VALSERGLUGLUSERGLYGLNGLNVALLEU
5   TRPVALPROASPGLUARGLEUALAGLUGLN
6   VALARGGLULEUTYRARGARGTYRPROGLU
7   GLYASPPROGLNALATHRLEUGLUALAALA

Entity 2, C16PN - C16PN - 407.568 Da.

Samples:

sample_1: NRho, [U-15N], 1 mM; C16PN 20 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%

sample_2: NRho, [U-15N], 0.8 mM; C16PN 20 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%

sample_3: NRho, [U-15N], 0.6 mM; C16PN 20 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%

sample_4: NRho, [U-15N], 0.5 mM; C16PN 20 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%

sample_5: NRho, [U-15N], 0.4 mM; C16PN 20 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%

sample_6: NRho, [U-15N], 0.3 mM; C16PN 20 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%

sample_7: NRho, [U-15N], 0.2 mM; C16PN 20 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%

sample_8: NRho, [U-15N], 0.1 mM; C16PN 20 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%

sample_9: NRho, [U-15N], 1 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%

sample_10: C16PN 20 mM; TRIS 20 mM; sodium chloride 50 mM; H2O 100%

sample_conditions_1: temperature: 298 K; pH: 5.5; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_4isotropicsample_conditions_1
2D 1H-15N HSQCsample_5isotropicsample_conditions_1
2D 1H-15N HSQCsample_6isotropicsample_conditions_1
2D 1H-15N HSQCsample_7isotropicsample_conditions_1
2D 1H-15N HSQCsample_8isotropicsample_conditions_1
2D 1H-15N HSQCsample_9isotropicsample_conditions_1
2D 1H-15N HSQCsample_10isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAK91039 BAP22307 BAP49934 BAQ38821 BAR66878
EMBL CAW26702 CCQ87103 CDH70226 CDH76316 CDI91489
GB AAG06474 ABJ12318 ABR81084 AEO74363 AFM64074
REF NP_251776 WP_003091333 WP_003104987 WP_003110073 WP_003138531