BMRB Entry 16313

Name: NMR Solution Structure of a Tubulin folding cofactor B obtained from Arabidopsis thaliana: Northeast Structural Genomics Consortium target AR3436A
Published: 2009-10-20

Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16313

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Solution Structure of a Tubulin folding cofactor B obtained from Arabidopsis thaliana: Northeast Structural Genomics Consortium target AR3436A

Deposition date:

2009-05-22

Original release date:

2009-10-20

Authors:

Mani, Rajeswari; Gurla, Swapna; Shastry, Ritu; Foote, Erica; Ciccosanti, Colleen; Jiang, Mei; Xiao, Rong; Nair, Rajesh; Everett, John; Huang, Yuanpeng; Acton, Tom; Rost, Burkhard; Montelione, Gaetano

Citation:

Citation: Mani, Rajeswari; Gurla, Swapna; Shastry, Ritu; Foote, Erica; Ciccosanti, Colleen; Jiang, Mei; Xiao, Rong; Nair, Rajesh; Everett, John; Huang, Yuanpeng; Acton, Tom; Rost, Burkhard; Montelione, Gaetano. "NMR Solution Structure of Tbulin folding Cofactor B obtained from Arabidopsis thaliana: Northeast Structural Genomics Consortium" .

Assembly members:

Assembly members:
AR3436A, polymer, 97 residues, 10852.224 Da.

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 21-23C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AR3436A: MGHHHHHHSHGDDSVHLHIT HANLKSFSADARFSPQMSVE AVKEKLWKKCGTSVNSMALE LYDDSGSKVAVLSDDSRPLG FFSPFDGFRLHIIDLDP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	366
15N chemical shifts	86
1H chemical shifts	551

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AR3436A	1

Entities:

Entity 1, AR3436A 97 residues - 10852.224 Da.

1-10 residues - MGHHHHHHSH is a N-terminal tag

1

MET

GLY

HIS

SER

HIS

2

GLY

ASP

SER

VAL

HIS

LEU

HIS

ILE

THR

3

HIS

ALA

ASN

LEU

LYS

SER

PHE

SER

ALA

ASP

4

ALA

ARG

PHE

SER

PRO

GLN

MET

SER

VAL

GLU

5

ALA

VAL

LYS

GLU

LYS

LEU

TRP

LYS

CYS

6

GLY

THR

SER

VAL

ASN

SER

MET

ALA

LEU

GLU

7

LEU

TYR

ASP

SER

GLY

SER

LYS

VAL

ALA

8

VAL

LEU

SER

ASP

SER

ARG

PRO

LEU

GLY

9

PHE

SER

PRO

PHE

ASP

GLY

PHE

ARG

LEU

10

HIS

ILE

ASP

LEU

ASP

PRO

Samples:

sample_1: AR3436A, [U-100% 13C; U-100% 15N], 0.78 mM; H2O 95%; D2O 5%; NaN3 0.02%; DTT 100 mM; CaCl2 5 mM; NaCl 10 mM; MES 20 mM

sample_2: AR3436A, [U-100% 13C; U-100% 15N], 0.95 mM; H2O 95%; D2O 5%; NaN3 0.02%; DTT 100 mM; CaCl2 5 mM; NaCl 10 mM; MES 20 mM

sample_3: AR3436A, [U-100% 13C; U-100% 15N], 0.4 mM; H2O 95%; D2O 5%; NaN3 0.02%; DTT 100 mM; CaCl2 5 mM; NaCl 10 mM; MES 20 mM

sample_4: AR3436A, [U-10% 13C; U-100% 15N], 0.56 mM; H2O 95%; D2O 5%; NaN3 0.02%; DTT 100 mM; CaCl2 5 mM; NaCl 10 mM; MES 20 mM

sample_conditions_1: ionic strength: 15 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_3	isotropic	sample_conditions_1
3D 1H-13Caliphatic NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C aromatic NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_3	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_1
(4,3)D GFT-HNNCABCA	sample_3	isotropic	sample_conditions_1
(4,3)D GFT-CABCACA(CO)NHN	sample_3	isotropic	sample_conditions_1
3D HNCO	sample_3	isotropic	sample_conditions_1
3D HNCA	sample_3	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HNHA	sample_4	isotropic	sample_conditions_1
3D H(CCO)NH	sample_3	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_3	isotropic	sample_conditions_1
(4,3)D GFT-HABCAB(CO)NHN	sample_3	isotropic	sample_conditions_1
3D C(CO)NH-TOCSY	sample_3	isotropic	sample_conditions_1
3D CCH-TOCSY	sample_3	isotropic	sample_conditions_1

Software:

CNS v2.0.6, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

AutoStruct v2.2.1, Huang, Tejero, Powers and Montelione - structure solution

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

AutoAssign v2.4.0, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz
Varian INOVA 600 MHz
Varian INOVA 500 MHz

PDB	2KJ6
DBJ	BAD43946 BAD44029 BAD44467
GB	AAF02820 AAM22958 AEE74873
REF	NP_187633
SP	Q67Z52
AlphaFold	Q67Z52