BMRB Entry 16312

Assembly:

Entities:

Entity 1, NmR46C 116 residues - 13400.581 Da.

L46 is a sequence variation; Please add a "remark 999"

Samples:

NC: NmR46C, [U-98% 13C; U-98% 15N], 1.2 mM; D2O 10%; H2O 90%; MES 20 mM; sodium chloride 200 mM; calcium chloride 5 mM; DTT 10 mM; DSS 50 uM; protease inhibitors 1x v/v; sodium azide 0.02%

NC5: NmR46C, [U-5% 13C; U-98% 15N], 1.2 mM; D2O 10%; H2O 90%; MES 20 mM; sodium chloride 200 mM; calcium chloride 5 mM; DTT 10 mM; DSS 50 uM; protease inhibitors 1x v/v; sodium azide 0.02%

NC5ani: NmR46C, [U-5% 13C; U-98% 15N], 1.2 mM; D2O 10%; H2O 90%; MES 20 mM; sodium chloride 200 mM; calcium chloride 5 mM; DTT 10 mM; DSS 50 uM; protease inhibitors 1x v/v; sodium azide 0.02%

sample_conditions_1: ionic strength: 430 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPSCAN, Glaser - processing

XEASY, Bartels et al. - chemical shift assignment, data analysis, peak picking

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

TALOS, Cornilescu, Delaglio and Bax - geometry optimization

CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, refinement, structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

Molmol, Koradi, Billeter and Wuthrich - data analysis, refinement, structure solution

AutoStruct, Huang, Tejero, Powers and Montelione - chemical shift assignment, refinement, structure solution

PSVS, Bhattacharya and Montelione - refinement

NMR spectrometers:

• Varian INOVA 750 MHz
• Varian INOVA 600 MHz