BMRB Entry 16297

Name: AIDA-1 SAM domain tandem
Published: 2009-09-04

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PDB ID: 2kiv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16297
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

AIDA-1 SAM domain tandem

Deposition date:

2009-05-12

Original release date:

2009-09-04

Authors:

Donaldson, Logan; Kurabi, Arwa

Citation:

Citation: Kurabi, Arwa; Brener, Stacey; Mobli, Mehdi; Kwan, Jamie; Donaldson, Logan. "A Nuclear Localization Signal at the SAM-SAM Domain Interface of AIDA-1 Suggests a Requirement for Domain Uncoupling Prior to Nuclear Import" J. Mol. Biol. 392, 1168-1177 (2009).
PubMed: 19666031

Assembly members:

Assembly members:
AIDA-1 SAM domain, polymer, 148 residues, 15101.473 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJexpress401

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AIDA-1 SAM domain: MHHHHHHLVPRGSVQTVGQW LESIGLPQYENHLMANGFDN VQAMGSNVMEDQDLLEIGIL NSGHRQRILQAIQLLPKMRP IGHDGAHPTSVAEWLDSIEL GDYTKAFLINGYTSMDLLKK IAEVELINVLKINLIGHRKR ILASLGDR

Data sets:

assigned_chemical_shifts
- assignments_aida6

Data type	Count
13C chemical shifts	510
15N chemical shifts	121
1H chemical shifts	791

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AIDA-1 SAM domain	1

Entities:

Entity 1, AIDA-1 SAM domain 148 residues - 15101.473 Da.

1

MET

HIS

LEU

VAL

PRO

2

ARG

GLY

SER

VAL

GLN

THR

VAL

GLY

GLN

TRP

3

LEU

GLU

SER

ILE

GLY

LEU

PRO

GLN

TYR

GLU

4

ASN

HIS

LEU

MET

ALA

ASN

GLY

PHE

ASP

ASN

5

VAL

GLN

ALA

MET

GLY

SER

ASN

VAL

MET

GLU

6

ASP

GLN

ASP

LEU

GLU

ILE

GLY

ILE

LEU

7

ASN

SER

GLY

HIS

ARG

GLN

ARG

ILE

LEU

GLN

8

ALA

ILE

GLN

LEU

PRO

LYS

MET

ARG

PRO

9

ILE

GLY

HIS

ASP

GLY

ALA

HIS

PRO

THR

SER

10

VAL

ALA

GLU

TRP

LEU

ASP

SER

ILE

GLU

LEU

11

GLY

ASP

TYR

THR

LYS

ALA

PHE

LEU

ILE

ASN

12

GLY

TYR

THR

SER

MET

ASP

LEU

LYS

13

ILE

ALA

GLU

VAL

GLU

LEU

ILE

ASN

VAL

LEU

14

LYS

ILE

ASN

LEU

ILE

GLY

HIS

ARG

LYS

ARG

15

ILE

LEU

ALA

SER

LEU

GLY

ASP

ARG

Samples:

sample_1: entity, [U-99% 13C; U-99% 15N], 1.1 mM; D2O 10%; H2O 90%; sodium azide 0.05%; sodium phosphate 5 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

Varian Uniform NMR System 600 MHz
Bruker Avance 900 MHz

PDB	2KIV
GB	AIC60436