BMRB Entry 16283

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PDB ID: 2kim
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16283
MolProbity Validation Chart

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Title:
1.7-mm microcryoprobe solution NMR structure of an O6-methylguanine DNA methyltransferase family protein from Vibrio parahaemolyticus. Northeast Structural Genomics Consortium target VpR247.
Deposition date:
2009-05-07
Original release date:
2010-09-17
Authors:
Aramini, James; Belote, Rachel; Ciccosanti, Colleen; Jiang, Mei; Rost, Burkhard; Nair, Rajesh; Swapna, G.; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano
Citation:

Citation: Aramini, James; Belote, Rachel; Ciccosanti, Colleen; Jiang, Mei; Rost, Burkhard; Nair, Rajesh; Swapna, G.; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano. "Solution NMR structure of an O6-methylguanine DNA methyltransferase family protein from Vibrio parahaemolyticus. Northeast Structural Genomics Consortium target VpR247."  .

Assembly members:

Assembly members:
VpR247, polymer, 108 residues, 12341.439 Da.

Natural source:

Natural source:   Common Name: Vibrio parahaemolyticus   Taxonomy ID: 670   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Vibrio parahaemolyticus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: VpR247-21.9

Entity Sequences (FASTA):

Entity Sequences (FASTA):
VpR247: MDQFLVQIFAVIHQIPKGKV STYGEIAKMAGYPGYARHVG KALGNLPEGSKLPWFRVINS QGKISLKGRDLDRQKQKLEA EGIEVSEIGKIALRKYKWQP LEHHHHHH

Data sets:
Data typeCount
13C chemical shifts485
15N chemical shifts104
1H chemical shifts768

Time Domain Data

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1VpR2471

Entities:

Entity 1, VpR247 108 residues - 12341.439 Da.

1   METASPGLNPHELEUVALGLNILEPHEALA
2   VALILEHISGLNILEPROLYSGLYLYSVAL
3   SERTHRTYRGLYGLUILEALALYSMETALA
4   GLYTYRPROGLYTYRALAARGHISVALGLY
5   LYSALALEUGLYASNLEUPROGLUGLYSER
6   LYSLEUPROTRPPHEARGVALILEASNSER
7   GLNGLYLYSILESERLEULYSGLYARGASP
8   LEUASPARGGLNLYSGLNLYSLEUGLUALA
9   GLUGLYILEGLUVALSERGLUILEGLYLYS
10   ILEALALEUARGLYSTYRLYSTRPGLNPRO
11   LEUGLUHISHISHISHISHISHIS

Related Database Links:

BMRB 16272
PDB 2KIF 2KIM
DBJ BAC59214
GB AGB09412 AGQ90485 AGQ98830 AHI99009 AKU55569
REF NP_797330 WP_005461131 WP_005481834 WP_021449579 WP_025501692
SP A6B4U8
AlphaFold A6B4U8

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