BMRB Entry 16277

Assembly:

Entities:

Entity 1, Actd 119 residues - Formula weight is not available

Residues GSFTM at the N-terminus represent a non-native tag and they have been removed from the coordinates file

Samples:

sample_1: Actd 0.2-0.3 ± 0.05 mM; H2O 90%; D2O 10%; phosphate 50 mM; arginine 50 mM; glutamate 50 mM; DTT 1 mM

sample_3: Actd, [U-13C; U-15N], 0.2-0.3 ± 0.05 mM; H2O 90%; D2O 10%; phosphate 50 mM; arginine 50 mM; glutamate 50 mM; DTT 1 mM

sample_2: Actd, [U-15N], 0.2-0.3 ± 0.05 mM; H2O 90%; D2O 10%; phosphate 50 mM; arginine 50 mM; glutamate 50 mM; DTT 1 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment

XEASY, Bartels et al. - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

• Bruker Avance 500 MHz
• Bruker Avance 800 MHz
• Bruker Avance 900 MHz