BMRB Entry 16271

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PDB ID: 2kie
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16271
MolProbity Validation Chart

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Title:
A PH domain within OCRL bridges clathrin mediated membrane trafficking to phosphoinositide metabolis
Deposition date:
2009-05-03
Original release date:
2009-06-25
Authors:
Mao, Yuxin; Balkin, Daniel; Zoncu, Roberto; Erdmann, Kai; Tomasini, Livia; Hu, Fenghua; Jin, Moonsoon; Hodsdon, Michael; De Camilli, Pietro
Citation:

Citation: Mao, Yuxin; Balkin, Daniel; Zoncu, Roberto; Erdmann, Kai; Tomasini, Livia; Hu, Fenghua; Jin, Moonsoon; Hodsdon, Michael; De Camilli, Pietro. "A PH domain within OCRL bridges clathrin-mediated membrane trafficking to phosphoinositide metabolism"  EMBO J. 28, 1831-1842 (2009).
PubMed: 19536138

Assembly members:

Assembly members:
PH domain, polymer, 372 residues, 41573.961 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX6p-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PH domain: GPLGSMEPPLPVGAQPLATV EGMEMKGPLREPCALTLAQR NGQYELIIQLHEKEQHVQDI IPINSHFRCVQEAEETLLID IASNSGCKIRVQGDWIRERR FEIPDEEHCLKFLSAVLAAQ KAQSGPLGSMEPPLPVGAQP LATVEGMEMKGPLREPCALT LAQRNGQYELIIQLHEKEQH VQDIIPINSHFRCVQEAEET LLIDIASNSGCKIRVQGDWI RERRFEIPDEEHCLKFLSAV LAAQKAQSGPLGSMEPPLPV GAQPLATVEGMEMKGPLREP CALTLAQRNGQYELIIQLHE KEQHVQDIIPINSHFRCVQE AEETLLIDIASNSGCKIRVQ GDWIRERRFEIPDEEHCLKF LSAVLAAQKAQS

Data sets:
Data typeCount
13C chemical shifts424
15N chemical shifts131
1H chemical shifts899

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1PH domain1

Entities:

Entity 1, PH domain 372 residues - 41573.961 Da.

1   GLYPROLEUGLYSERMETGLUPROPROLEU
2   PROVALGLYALAGLNPROLEUALATHRVAL
3   GLUGLYMETGLUMETLYSGLYPROLEUARG
4   GLUPROCYSALALEUTHRLEUALAGLNARG
5   ASNGLYGLNTYRGLULEUILEILEGLNLEU
6   HISGLULYSGLUGLNHISVALGLNASPILE
7   ILEPROILEASNSERHISPHEARGCYSVAL
8   GLNGLUALAGLUGLUTHRLEULEUILEASP
9   ILEALASERASNSERGLYCYSLYSILEARG
10   VALGLNGLYASPTRPILEARGGLUARGARG
11   PHEGLUILEPROASPGLUGLUHISCYSLEU
12   LYSPHELEUSERALAVALLEUALAALAGLN
13   LYSALAGLNSERGLYPROLEUGLYSERMET
14   GLUPROPROLEUPROVALGLYALAGLNPRO
15   LEUALATHRVALGLUGLYMETGLUMETLYS
16   GLYPROLEUARGGLUPROCYSALALEUTHR
17   LEUALAGLNARGASNGLYGLNTYRGLULEU
18   ILEILEGLNLEUHISGLULYSGLUGLNHIS
19   VALGLNASPILEILEPROILEASNSERHIS
20   PHEARGCYSVALGLNGLUALAGLUGLUTHR
21   LEULEUILEASPILEALASERASNSERGLY
22   CYSLYSILEARGVALGLNGLYASPTRPILE
23   ARGGLUARGARGPHEGLUILEPROASPGLU
24   GLUHISCYSLEULYSPHELEUSERALAVAL
25   LEUALAALAGLNLYSALAGLNSERGLYPRO
26   LEUGLYSERMETGLUPROPROLEUPROVAL
27   GLYALAGLNPROLEUALATHRVALGLUGLY
28   METGLUMETLYSGLYPROLEUARGGLUPRO
29   CYSALALEUTHRLEUALAGLNARGASNGLY
30   GLNTYRGLULEUILEILEGLNLEUHISGLU
31   LYSGLUGLNHISVALGLNASPILEILEPRO
32   ILEASNSERHISPHEARGCYSVALGLNGLU
33   ALAGLUGLUTHRLEULEUILEASPILEALA
34   SERASNSERGLYCYSLYSILEARGVALGLN
35   GLYASPTRPILEARGGLUARGARGPHEGLU
36   ILEPROASPGLUGLUHISCYSLEULYSPHE
37   LEUSERALAVALLEUALAALAGLNLYSALA
38   GLNSER

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