BMRB Entry 16256

Name: Drosha RNA Binding Domain
Published: 2010-04-01

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PDB ID: 2khx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16256
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Drosha RNA Binding Domain

Deposition date:

2009-04-13

Original release date:

2010-04-01

Authors:

Mueller, Geoffrey; Miller, Matthew; Ghosh, Mahua; DeRose, Eugene; London, Robert; Hall, Traci

Citation:

Citation: Mueller, Geoffrey; Miller, Matthew; DeRose, Eugene; Ghosh, Mahua; London, Robert; Hall, Traci. "Solution structure of the Drosha double-stranded RNA-binding domain" Silence 1, 2-. (2010).
PubMed: 20226070

Assembly members:

Assembly members:
binding domain, polymer, 90 residues, 8761.069 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet21c

Entity Sequences (FASTA):

Entity Sequences (FASTA):
binding domain: GSPQFMLNQDWNDPKSQLQQ CCLTLRTEGKEPDIPLYKTL QTVGPSHARTYTVAVYFKGE RIGCGKGPSIQQAEMGAAMD ALEKYNFPQM

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	244
15N chemical shifts	68
1H chemical shifts	473

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	binding domain	1

Entities:

Entity 1, binding domain 90 residues - 8761.069 Da.

1	GLY	SER	PRO	GLN	PHE	MET	LEU	ASN	GLN	ASP
2	TRP	ASN	ASP	PRO	LYS	SER	GLN	LEU	GLN	GLN
3	CYS	CYS	LEU	THR	LEU	ARG	THR	GLU	GLY	LYS
4	GLU	PRO	ASP	ILE	PRO	LEU	TYR	LYS	THR	LEU
5	GLN	THR	VAL	GLY	PRO	SER	HIS	ALA	ARG	THR
6	TYR	THR	VAL	ALA	VAL	TYR	PHE	LYS	GLY	GLU
7	ARG	ILE	GLY	CYS	GLY	LYS	GLY	PRO	SER	ILE
8	GLN	GLN	ALA	GLU	MET	GLY	ALA	ALA	MET	ASP
9	ALA	LEU	GLU	LYS	TYR	ASN	PHE	PRO	GLN	MET

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
CN-4D simultaneous NOESY	sample_1	isotropic	sample_conditions_1
HB-HE/HD-aromatic	sample_1	isotropic	sample_conditions_1

PDB	2KHX
DBJ	BAA91511 BAB22917 BAE25729 BAE28629 BAO93839
EMBL	CAG32380 CBN82191 CDQ74551
GB	AAD29637 AAF80558 AAH41162 AAH50057 AAH55696
REF	NP_001006379 NP_001093882 NP_001101125 NP_001103942 NP_001107152
SP	Q5HZJ0 Q9NRR4
TPG	DAA17862 DAA17863
AlphaFold	Q5HZJ0 Q9NRR4

BMRB Entry 16256

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	SER	PRO	GLN	PHE	MET	LEU	ASN	GLN	ASP
2	TRP	ASN	ASP	PRO	LYS	SER	GLN	LEU	GLN	GLN
3	CYS	CYS	LEU	THR	LEU	ARG	THR	GLU	GLY	LYS
4	GLU	PRO	ASP	ILE	PRO	LEU	TYR	LYS	THR	LEU
5	GLN	THR	VAL	GLY	PRO	SER	HIS	ALA	ARG	THR
6	TYR	THR	VAL	ALA	VAL	TYR	PHE	LYS	GLY	GLU
7	ARG	ILE	GLY	CYS	GLY	LYS	GLY	PRO	SER	ILE
8	GLN	GLN	ALA	GLU	MET	GLY	ALA	ALA	MET	ASP
9	ALA	LEU	GLU	LYS	TYR	ASN	PHE	PRO	GLN	MET

1	GLY	SER	PRO	GLN	PHE	MET	LEU	ASN	GLN	ASP
2	TRP	ASN	ASP	PRO	LYS	SER	GLN	LEU	GLN	GLN
3	CYS	CYS	LEU	THR	LEU	ARG	THR	GLU	GLY	LYS
4	GLU	PRO	ASP	ILE	PRO	LEU	TYR	LYS	THR	LEU
5	GLN	THR	VAL	GLY	PRO	SER	HIS	ALA	ARG	THR
6	TYR	THR	VAL	ALA	VAL	TYR	PHE	LYS	GLY	GLU
7	ARG	ILE	GLY	CYS	GLY	LYS	GLY	PRO	SER	ILE
8	GLN	GLN	ALA	GLU	MET	GLY	ALA	ALA	MET	ASP
9	ALA	LEU	GLU	LYS	TYR	ASN	PHE	PRO	GLN	MET

1	GLY	SER	PRO	GLN	PHE	MET	LEU	ASN	GLN	ASP
2	TRP	ASN	ASP	PRO	LYS	SER	GLN	LEU	GLN	GLN
3	CYS	CYS	LEU	THR	LEU	ARG	THR	GLU	GLY	LYS
4	GLU	PRO	ASP	ILE	PRO	LEU	TYR	LYS	THR	LEU
5	GLN	THR	VAL	GLY	PRO	SER	HIS	ALA	ARG	THR
6	TYR	THR	VAL	ALA	VAL	TYR	PHE	LYS	GLY	GLU
7	ARG	ILE	GLY	CYS	GLY	LYS	GLY	PRO	SER	ILE
8	GLN	GLN	ALA	GLU	MET	GLY	ALA	ALA	MET	ASP
9	ALA	LEU	GLU	LYS	TYR	ASN	PHE	PRO	GLN	MET