BMRB Entry 16246
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PDB ID: 2kh9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16246
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Citation: Martin-Tumasz, Stephen; Butcher, Samuel. "(1)H, (13)C and (15)N resonance assignments of a ribonucleoprotein complex consisting of Prp24-RRM2 bound to a fragment of U6 RNA." Biomol. NMR Assignments 3, 227-230 (2009).
PubMed: 19693704
Assembly members:
Prp24-RRM2, polymer, 92 residues, 10012.586 Da.
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21-b
Entity Sequences (FASTA):
Prp24-RRM2: MTECTLWMTNFPPSYTQRNI
RDLLQDINVVALSIRLPSLR
FNTSRRFAYIDVTSKEDARY
CVEKLNGLKIEGYTLVTKVS
NPLELEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 257 |
| 15N chemical shifts | 83 |
| 1H chemical shifts | 522 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Prp24-RRM2 | 1 |
Entities:
Entity 1, Prp24-RRM2 92 residues - 10012.586 Da.
Final 8 residues are non-native due to 6x His tag
| 1 | MET | THR | GLU | CYS | THR | LEU | TRP | MET | THR | ASN | ||||
| 2 | PHE | PRO | PRO | SER | TYR | THR | GLN | ARG | ASN | ILE | ||||
| 3 | ARG | ASP | LEU | LEU | GLN | ASP | ILE | ASN | VAL | VAL | ||||
| 4 | ALA | LEU | SER | ILE | ARG | LEU | PRO | SER | LEU | ARG | ||||
| 5 | PHE | ASN | THR | SER | ARG | ARG | PHE | ALA | TYR | ILE | ||||
| 6 | ASP | VAL | THR | SER | LYS | GLU | ASP | ALA | ARG | TYR | ||||
| 7 | CYS | VAL | GLU | LYS | LEU | ASN | GLY | LEU | LYS | ILE | ||||
| 8 | GLU | GLY | TYR | THR | LEU | VAL | THR | LYS | VAL | SER | ||||
| 9 | ASN | PRO | LEU | GLU | LEU | GLU | HIS | HIS | HIS | HIS | ||||
| 10 | HIS | HIS |
Related Database Links:
| BMRB | 16230 16243 16244 |
| PDB | 2GHP 2GO9 2KH9 4N0T |
| DBJ | GAA25719 |
| EMBL | CAA89251 CAY82100 |
| GB | AAU09775 AHY76723 AJP40962 AJS62134 AJS62569 |
| REF | NP_013995 |
| SP | P49960 |
| TPG | DAA10168 |
| AlphaFold | P49960 |
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