BMRB Entry 16165

Name: 1H, 15N and 13C backbone resonance assignments of domain 2 (D2) of the non-structural 5A protein (NS5A) from the JFH1 Hepatitis C virus (HCV) strain.
Published: 2009-03-20

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16165

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C backbone resonance assignments of domain 2 (D2) of the non-structural 5A protein (NS5A) from the JFH1 Hepatitis C virus (HCV) strain.

Deposition date:

2009-02-11

Original release date:

2009-03-20

Authors:

Hanoulle, Xavier; Badillo, Aurelie; Wieruszeski, Jean-Michel; Verdegem, Dries; Landrieu, Isabelle; Bartenschlager, Ralf; Penin, Francois; Lippens, Guy

Citation:

Citation: Hanoulle, Xavier; Badillo, Aurelie; Wieruszeski, Jean-Michel; Verdegem, Dries; Landrieu, Isabelle; Bartenschlager, Ralf; Penin, Francois; Lippens, Guy. "Hepatitis C Virus NS5A protein is a substrate for the Peptidyl-Prolyl cis/trans Isomerase activity of Cyclophilins A and B." J. Biol. Chem. ., .-. (2009).
PubMed: 19297321

Assembly members:

Assembly members:
HCV_(JFH1)_NS5A-D2, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Hepatitis C Virus Taxonomy ID: 11103 Superkingdom: not available Kingdom: not available Genus/species: Hepacivirus Hepatitis C Virus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pT7.7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HCV_(JFH1)_NS5A-D2: MNTYDVDMVDANLLMEGGVA QTEPESRVPVLDFLEPMAEE ESDLEPSIPSECMLPRSGFP RALPAWARPDYNPPLVESWR RPDYQPPTVAGCALPLQHHH HHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	277
15N chemical shifts	80
1H chemical shifts	80

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HCV (JFH1) NS5A-D2	1

Entities:

Entity 1, HCV (JFH1) NS5A-D2 103 residues - Formula weight is not available

The first Methionine residue is a cloning artifact. The last 8 residues represent a non-native affinity tag. This is the domain 2 of the HCV (JFH1, genotype 2a) NS5A protein.

1

MET

ASN

THR

TYR

ASP

VAL

ASP

MET

VAL

ASP

2

ALA

ASN

LEU

MET

GLU

GLY

VAL

ALA

3

GLN

THR

GLU

PRO

GLU

SER

ARG

VAL

PRO

VAL

4

LEU

ASP

PHE

LEU

GLU

PRO

MET

ALA

GLU

5

GLU

SER

ASP

LEU

GLU

PRO

SER

ILE

PRO

SER

6

GLU

CYS

MET

LEU

PRO

ARG

SER

GLY

PHE

PRO

7

ARG

ALA

LEU

PRO

ALA

TRP

ALA

ARG

PRO

ASP

8

TYR

ASN

PRO

LEU

VAL

GLU

SER

TRP

ARG

9

ARG

PRO

ASP

TYR

GLN

PRO

THR

VAL

ALA

10

GLY

CYS

ALA

LEU

PRO

LEU

GLN

HIS

11

HIS

Samples:

sample_1: HCV (JFH1) NS5A-D2, [U-95% 13C; U-98% 15N], 350 uM; sodium phosphate 20 mM; sodium chloride 30 mM; DTT 1 mM; sodium azide 0.02%; D2O 5%; H2O 95%

sample_conditions_1: ionic strength: 30 mM; pH: 6.4; pressure: 1.0 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCANNH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - collection, processing

In_house_product_plane_algorithm, Dries Verdegem & Guy Lippens - chemical shift assignment, data analysis

NMR spectrometers:

Bruker Avance 600 MHz

Swiss-Prot	Q99IB8
Genbank	AB047639
euHCVdb	AB047639
DBJ	BAB32872 BAD06942 BAF34893
GB	ABX82715 ABY68009 ABY68010 ABY68011 ABY68012
SP	Q99IB8
AlphaFold	Q99IB8