BMRB Entry 16123

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16123

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for d+PHS/V66K SNase
Deposition date:
2009-01-16
Original release date:
2012-08-07
Authors:
Chimenti, Michael
Citation:

Citation: Chimenti, Michael; Castaneda, Carlos; Majumdar, Ananya; Garcia-Moreno, Bertrand. "Structural origins of high apparent dielectric constants experienced by ionizable groups in the hydrophobic core of a protein"  J. Mol. Biol. 405, 361-377 (2011).
PubMed: 21059359

Assembly members:

Assembly members:
D+PHS-V66K_Nuclease, polymer, 143 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24a+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
D+PHS-V66K_Nuclease: ATSTKKLHKEPATLIKAIDG DTVKLMYKGQPMTFRLLLVD TPEFNEKYGPEASAFTKKMK ENAKKIEVEFDKGQRTDKYG RGLAYIYADGKMVNEALVRQ GLAKVAYVYKGNNTHEQLLR KAEAQAKKEKLNIWSEDNAD SGQ

Data sets:
Data typeCount
13C chemical shifts243
15N chemical shifts130
1H chemical shifts130

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1D+PHS/V66K1

Entities:

Entity 1, D+PHS/V66K 143 residues - Formula weight is not available

Residues 44-49 (an active site loop) were deleted from the protein; the original numbering scheme was kept.

1   ALATHRSERTHRLYSLYSLEUHISLYSGLU
2   PROALATHRLEUILELYSALAILEASPGLY
3   ASPTHRVALLYSLEUMETTYRLYSGLYGLN
4   PROMETTHRPHEARGLEULEULEUVALASP
5   THRPROGLUPHEASNGLULYSTYRGLYPRO
6   GLUALASERALAPHETHRLYSLYSMETLYS
7   GLUASNALALYSLYSILEGLUVALGLUPHE
8   ASPLYSGLYGLNARGTHRASPLYSTYRGLY
9   ARGGLYLEUALATYRILETYRALAASPGLY
10   LYSMETVALASNGLUALALEUVALARGGLN
11   GLYLEUALALYSVALALATYRVALTYRLYS
12   GLYASNASNTHRHISGLUGLNLEULEUARG
13   LYSALAGLUALAGLNALALYSLYSGLULYS
14   LEUASNILETRPSERGLUASPASNALAASP
15   SERGLYGLN

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks