BMRB Entry 16053

Title:
Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method
Deposition date:
2008-12-07
Original release date:
2009-03-20
Authors:
Kumeta, Hiroyuki; Kobashigawa, Yoshinori; Ogura, Kenji; Inagaki, Fuyuhiko
Citation:

Citation: Kobashigawa, Yoshinori; Kumeta, Hiroyuki; Ogura, Kenji; Inagaki, Fuyuhiko. "Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method"  J. Biomol. NMR 43, 145-150 (2009).
PubMed: 19140010

Assembly members:

Assembly members:
entity, polymer, 141 residues, 8004.950 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Data sets:
Data typeCount
13C chemical shifts305
15N chemical shifts74
1H chemical shifts483

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 141 residues - 8004.950 Da.

1   GLYPROGLYTHRPHEGLYTHRALALYSALA
2   ARGTYRASPPHECYSALAARGASPARGSER
3   GLULEUSERLEULYSGLUGLYASPILEILE
4   LYSILELEUASNLYSLYSGLYGLNGLNGLY
5   TRPTRPARGGLYGLUILETYRGLYARGILE
6   GLYTRPPHEPROSERASNTYRVALGLUGLU
7   ASPTYRSERGLUTYRLEUPROGLUTHRGLY
8   GLYGLYSERGLYSERSERMETGLUTYRLYS
9   LEUILELEUASNGLYLYSTHRLEULYSGLY
10   GLUTHRTHRTHRGLUALAVALASPALAALA
11   THRALAGLULYSVALPHELYSGLNTYRALA
12   ASNASPGLYVALASPGLYGLUTRPTHRTYR
13   ASPASPALATHRLYSTHRPHETHRVALTHR
14   GLUHISSERLEUGLUHISHISHISHISHIS
15   HIS

Related Database Links:

PDB 2KBT

Download HSQC peak lists in one of the following formats:
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