BMRB Entry 15966
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PDB ID: 2k73
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15966
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Zhou, Yunpeng; Cierpicki, Tomasz; Flores Jimenez, Ricardo; Lukasik, Stephen; Ellena, Jeffrey; Cafiso, David; Kadukura, Hiroshi; Beckwith, Jon; Bushweller, John. "NMR solution structure of the integral membrane enzyme DsbB: functional insights into DsbB-catalyzed disulfide bond formation." Mol. Cell 31, 896-908 (2008).
PubMed: 18922471
Assembly members:
Disulfide_bond_formation_protein_B, polymer, 183 residues, 20948.965 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b
Entity Sequences (FASTA):
Disulfide_bond_formation_protein_B: MLRFLNQASQGRGAWLLMAF
TALALELTALWFQHVMLLKP
CVLSIYERAALFGVLGAALI
GAIAPKTPLRYVAMVIWLYS
AFRGVQLTYEHTMLQLYPSP
FATSDFMVRFPEWLPLDKWV
PQVFVASGDCAERQWDFLGL
EMPQWLLGIFIAYLIVAVLV
VISQPFKAKKRDLFGRGHHH
HHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 499 |
| 15N chemical shifts | 170 |
| 1H chemical shifts | 170 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DsbB | 1 |
Entities:
Entity 1, DsbB 183 residues - 20948.965 Da.
residue 177-183 (-GHHHHHH) represents a non-native affinity tag and linker.
| 1 | MET | LEU | ARG | PHE | LEU | ASN | GLN | ALA | SER | GLN | ||||
| 2 | GLY | ARG | GLY | ALA | TRP | LEU | LEU | MET | ALA | PHE | ||||
| 3 | THR | ALA | LEU | ALA | LEU | GLU | LEU | THR | ALA | LEU | ||||
| 4 | TRP | PHE | GLN | HIS | VAL | MET | LEU | LEU | LYS | PRO | ||||
| 5 | CYS | VAL | LEU | SER | ILE | TYR | GLU | ARG | ALA | ALA | ||||
| 6 | LEU | PHE | GLY | VAL | LEU | GLY | ALA | ALA | LEU | ILE | ||||
| 7 | GLY | ALA | ILE | ALA | PRO | LYS | THR | PRO | LEU | ARG | ||||
| 8 | TYR | VAL | ALA | MET | VAL | ILE | TRP | LEU | TYR | SER | ||||
| 9 | ALA | PHE | ARG | GLY | VAL | GLN | LEU | THR | TYR | GLU | ||||
| 10 | HIS | THR | MET | LEU | GLN | LEU | TYR | PRO | SER | PRO | ||||
| 11 | PHE | ALA | THR | SER | ASP | PHE | MET | VAL | ARG | PHE | ||||
| 12 | PRO | GLU | TRP | LEU | PRO | LEU | ASP | LYS | TRP | VAL | ||||
| 13 | PRO | GLN | VAL | PHE | VAL | ALA | SER | GLY | ASP | CYS | ||||
| 14 | ALA | GLU | ARG | GLN | TRP | ASP | PHE | LEU | GLY | LEU | ||||
| 15 | GLU | MET | PRO | GLN | TRP | LEU | LEU | GLY | ILE | PHE | ||||
| 16 | ILE | ALA | TYR | LEU | ILE | VAL | ALA | VAL | LEU | VAL | ||||
| 17 | VAL | ILE | SER | GLN | PRO | PHE | LYS | ALA | LYS | LYS | ||||
| 18 | ARG | ASP | LEU | PHE | GLY | ARG | GLY | HIS | HIS | HIS | ||||
| 19 | HIS | HIS | HIS |
Related Database Links:
| BMRB | 18395 18493 |
| PDB | 2HI7 2K73 2K74 2LEG 2LTQ 2ZUP 2ZUQ 3E9J |
| DBJ | BAA36032 BAB35103 BAI24997 BAI30121 BAI35441 |
| EMBL | CAQ31687 CAQ98064 CAR12682 CAR18016 CAU97139 |
| GB | AAA23711 AAB25233 AAC74269 AAG56036 AAN42789 |
| PIR | H85696 |
| REF | NP_309707 NP_415703 NP_707082 WP_000652474 WP_000943442 |
| SP | P0A6M2 P0A6M3 Q0T5L6 Q31ZM6 Q32H31 |
| AlphaFold | P0A6M2 P0A6M3 Q0T5L6 Q31ZM6 Q32H31 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks