BMRB Entry 15913

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PDB ID: 2k78
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15913
MolProbity Validation Chart

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Title:
Solution Structure of the IsdC NEAT domain bound to Zinc Protoporphyrin
Deposition date:
2008-08-07
Original release date:
2008-08-14
Authors:
Villareal, Valerie; Pilpa, Rosemarie; Robson, Scott; Fadeev, Evgeny; Clubb, Robert
Citation:

Citation: Villareal, Valerie; Pilpa, Rosemarie; Robson, Scott; Fadeev, Evgeny; Clubb, Robert. "The IsdC protein from Staphylococcus aureus uses a flexible binding pocket to capture heme"  J. Biol. Chem. 283, 31591-31600 (2008).
PubMed: 18715872

Assembly members:

Assembly members:
IsdC, polymer, 147 residues, 14322.988 Da.
ZNH, non-polymer, 626.051 Da.

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IsdC: MGSSHHHHHHSSGLVPRGSH MSANAADSGTLNYEVYKYNT NDTSIANDYFNKPAKYIKKN GKLYVQITVNHSHWITGMSI EGHKENIISKNTAKDERTSE FEVSKLNGKIDGKIDVYIDE KVNGKPFKYDHHYNITYKFN GPTDVAG

Data sets:
Data typeCount
13C chemical shifts488
15N chemical shifts130
1H chemical shifts730

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1IsdC1
2ZNH2

Entities:

Entity 1, IsdC 147 residues - 14322.988 Da.

Residues 4-24 represent a Histidine-tag that was not included in the structure calculations

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METSERALAASNALAALAASPSERGLYTHR
4   LEUASNTYRGLUVALTYRLYSTYRASNTHR
5   ASNASPTHRSERILEALAASNASPTYRPHE
6   ASNLYSPROALALYSTYRILELYSLYSASN
7   GLYLYSLEUTYRVALGLNILETHRVALASN
8   HISSERHISTRPILETHRGLYMETSERILE
9   GLUGLYHISLYSGLUASNILEILESERLYS
10   ASNTHRALALYSASPGLUARGTHRSERGLU
11   PHEGLUVALSERLYSLEUASNGLYLYSILE
12   ASPGLYLYSILEASPVALTYRILEASPGLU
13   LYSVALASNGLYLYSPROPHELYSTYRASP
14   HISHISTYRASNILETHRTYRLYSPHEASN
15   GLYPROTHRASPVALALAGLY

Entity 2, ZNH - C34 H32 N4 O4 Zn - 626.051 Da.

1   ZNH

Related Database Links:

PDB 2K78 2O6P
DBJ BAB42227 BAB57293 BAB94878 BAF67314 BAF78005
EMBL CAG40106 CAG42839 CAI80683 CAQ49552 CBI49004
GB AAL33767 AAW38020 ABD20415 ABD30198 ABQ48990
REF WP_000789795 WP_000789808 WP_000789809 WP_000789810 WP_000789811
SP A5IS17 A6QG32 A6U0U8 A7X149 A8Z1R1
AlphaFold A5IS17 A6QG32 A6U0U8 A7X149 A8Z1R1

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