BMRB Entry 15911

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15911

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Title:
NMR backbone assignments of the periplasmic loop P2 of the MalF subunit of the maltose ATP binding cassette transporter
Deposition date:
2008-08-07
Original release date:
2008-10-02
Authors:
Jacso, Tomas; Grote, Mathias; Schmieder, Peter; Schneider, Erwin; Reif, Bernd
Citation:

Citation: Jacso, Tomas; Grote, Mathias; Daus, Martin; Schmieder, Peter; Keller, Sandro; Schneider, Erwin; Reif, Bernd. "The periplasmic loop P2 of the MalF subunit of the maltose ATP binding cassette transporter is sufficient to bind the maltose binding protein MalE"  Biochemistry 48, 2216-2225 (2009).
PubMed: 19159328

Assembly members:

Assembly members:
MalF-P2, polymer, 184 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MalF-P2: MNYSSTNQLTFERAQEVLLD RSWQAGKTYNFGLYPAGDEW QLALSDGETGKNYLSDAFKF GGEQKLQLKETTAQPEGERA NLRVITQNRQALSDITAILP DGNKVMMSSLRQFSGTQPLY TLDGDGTLTNNQSGVKYRPN NQIGFYQSITADGNWGDEKL SPGYTVTTGWKNFTRVFTDE GIQK

Data sets:
Data typeCount
13C chemical shifts329
15N chemical shifts157
1H chemical shifts157

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MalF-P21

Entities:

Entity 1, MalF-P2 184 residues - Formula weight is not available

The second residue in MalF-P2 is residue N93 in MalF

1   METASNTYRSERSERTHRASNGLNLEUTHR
2   PHEGLUARGALAGLNGLUVALLEULEUASP
3   ARGSERTRPGLNALAGLYLYSTHRTYRASN
4   PHEGLYLEUTYRPROALAGLYASPGLUTRP
5   GLNLEUALALEUSERASPGLYGLUTHRGLY
6   LYSASNTYRLEUSERASPALAPHELYSPHE
7   GLYGLYGLUGLNLYSLEUGLNLEULYSGLU
8   THRTHRALAGLNPROGLUGLYGLUARGALA
9   ASNLEUARGVALILETHRGLNASNARGGLN
10   ALALEUSERASPILETHRALAILELEUPRO
11   ASPGLYASNLYSVALMETMETSERSERLEU
12   ARGGLNPHESERGLYTHRGLNPROLEUTYR
13   THRLEUASPGLYASPGLYTHRLEUTHRASN
14   ASNGLNSERGLYVALLYSTYRARGPROASN
15   ASNGLNILEGLYPHETYRGLNSERILETHR
16   ALAASPGLYASNTRPGLYASPGLULYSLEU
17   SERPROGLYTYRTHRVALTHRTHRGLYTRP
18   LYSASNPHETHRARGVALPHETHRASPGLU
19   GLYILEGLNLYS

Related Database Links:

PDB 2R6G 3FH6 3PUV 3PUW 3PUX 3PUY 3PUZ 3PV0 3RLF 4JBW 4KHZ 4KI0
DBJ BAB38439 BAE78035 BAG79848 BAI57430 BAJ45745
EMBL CAP78493 CAQ34382 CAR01011 CAR05668 CAR10710
GB AAB59055 AAC43127 AAC77003 AAG59232 AAN45593
REF NP_313043 NP_418457 NP_709886 WP_001295280 WP_001296633
SP P02916 Q7A937 Q83P81 Q8FB38
AlphaFold P02916 Q7A937 Q83P81 Q8FB38

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks