BMRB Entry 15901

Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15901

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for D+PHS, a hyperstable variant of SNase, at pH 4.66
Deposition date:
2008-08-01
Original release date:
2009-06-24
Authors:
Castaneda, Carlos
Citation:

Citation: Castaneda, Carlos; Fitch, Carolyn; Majumdar, Ananya; Khangulov, Victor; Schlessman, Jamie; Garcia-Moreno, Bertrand. "Molecular determinants of the pKa values of Asp and Glu residues in staphylococcal nuclease."  Proteins 77, 570-588 (2009).
PubMed: 19533744

Assembly members:

Assembly members:
D+PHS, polymer, 143 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET24a+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
D+PHS: ATSTKKLHKEPATLIKAIDG DTVKLMYKGQPMTFRLLLVD TPEFNEKYGPEASAFTKKMV ENAKKIEVEFDKGQRTDKYG RGLAYIYADGKMVNEALVRQ GLAKVAYVYKGNNTHEQLLR KAEAQAKKEKLNIWSEDNAD SGQ

Data sets:
Data typeCount
13C chemical shifts276
15N chemical shifts138
1H chemical shifts138

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1D+PHS1

Entities:

Entity 1, D+PHS 143 residues - Formula weight is not available

1   ALATHRSERTHRLYSLYSLEUHISLYSGLU
2   PROALATHRLEUILELYSALAILEASPGLY
3   ASPTHRVALLYSLEUMETTYRLYSGLYGLN
4   PROMETTHRPHEARGLEULEULEUVALASP
5   THRPROGLUPHEASNGLULYSTYRGLYPRO
6   GLUALASERALAPHETHRLYSLYSMETVAL
7   GLUASNALALYSLYSILEGLUVALGLUPHE
8   ASPLYSGLYGLNARGTHRASPLYSTYRGLY
9   ARGGLYLEUALATYRILETYRALAASPGLY
10   LYSMETVALASNGLUALALEUVALARGGLN
11   GLYLEUALALYSVALALATYRVALTYRLYS
12   GLYASNASNTHRHISGLUGLNLEULEUARG
13   LYSALAGLUALAGLNALALYSLYSGLULYS
14   LEUASNILETRPSERGLUASPASNALAASP
15   SERGLYGLN

Related Database Links:

BMRB 16123
PDB 1TQO 1TR5 1TT2 2RBM 3BDC 3C1E 3C1F 3D4D 3D4W 3D8G 3DHQ 3E5S 3EJI 3ERO 3ERQ 3EVQ 3H6M 3HEJ 3HZX 3ITP 3LX0 3MEH 3MHB 3MVV 3MXP 3MZ5 3NHH 3NK9 3NP8 3NQT 3NXW 3OSO 3OWF 3P1H 3P75 3PMF 3QB3 3QOJ 3QOL 3QON 3R3O 3RUZ 3S9W 3SHL 3SK4 3SK5 3SK8 3SR1 3SXH 3T13 3TP6 3TP7 3TP8 3V2T 3VA5 4DF7 4DFA 4DGZ 4DU9 4EQN 4EQO 4EQP 4F7X 4F8M 4HMI 4HMJ 4HTH 4IAL 4IUN 4IZ8 4J1M 4J6H 4K2K 4K2L 4K5V 4KD3 4KHV 4KJN 4KJO 4KTA 4KV6 4KY5 4KY6 4KY7 4LAA 4ME5 4MIU 4N9P 4N9T 4NDX 4NKL 4NMZ 4NP5 4OL7 4PMB 4PMC 4PNY 4RKB 4RKL 4S3S 4TRD 4WRD 4YIJ 4ZQ3 4ZUI 4ZUJ 5C3W 5C3X 5C4H 5C4Z 5C5L 5C5M 5C6A 5CC4 5CGK 5CR3 5CV4 5CV5 5CV6 5CV7 5CV8 5CV9 5DAU 5DEH 5E1F 5E3F 5EGT 5EGZ

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks