BMRB Entry 15846

Assembly:

Entities:

Entity 1, entity 105 residues - 12458.204 Da.

The sequence used to solve the structure is a construct (21-117aa) of the full length BcR97. The construct has been designed eliminating the first 20 residues based on the disorder-prediction algorithms. The residues 21-117 have been numbered as 1-97 in the current entry. The 8 residues at the C-terminal, LEHHHHHH correspond to the purification tag.

Samples:

sample_1: BcR97A protein, [U-100% 13C; U-100% 15N], 1.25 ± 0.2 mM; DTT 10 mM; DSS 50 uM; NaN3 0.02%; CaCl2 5 mM; NaCl 100 uM; MES 20 mM; D2O, [U-2H], 10%; H2O 90%

sample_2: BcR97A protein, [U-10% 13C; U-100% 15N], 0.77 ± 0.2 mM; DTT 10 mM; DSS 50 uM; NaN3 0.02%; CaCl2 5 mM; NaCl 100 uM; MES 20 mM; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: ionic strength: 105 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Software:

AutoAssign v2.2.1, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

AutoStruct v2.2.1, Huang, Tejero, Powers and Montelione - chemical shift assignment, structure solution

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - geometry optimization, structure solution

CNS v2.0.6, Brunger, Adams, Clore, Gros, Nilges and Read - geometry optimization, refinement

NMR spectrometers:

• Varian INOVA 600 MHz
• Bruker Avance 600 MHz