BMRB Entry 15829

Name: SOLUTION NMR STRUCTURE OF SAG0934 from Streptococcus agalactiae. NORTHEAST STRUCTURAL GENOMICS TARGET SaR32[1-108].
Published: 2009-05-07

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PDB ID: 2k5d
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15829
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SOLUTION NMR STRUCTURE OF SAG0934 from Streptococcus agalactiae. NORTHEAST STRUCTURAL GENOMICS TARGET SaR32[1-108].

Deposition date:

2008-06-26

Original release date:

2009-05-07

Authors:

Aramini, James; Rossi, Paolo; Zhao, Li; Foote, Erica; Jiang, Mei; Xiao, Rong; Sharma, Seema; Swapna, G; Nair, Rajesh; Everett, John; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano

Citation:

Citation: Aramini, James; Rossi, Paolo; Zhao, Li; Foote, Erica; Jiang, Mei; Xiao, Rong; Sharma, Seema; Swapna, G; Nair, Rajesh; Everett, John; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano. "SOLUTION NMR STRUCTURE OF SAG0934 from Streptococcus agalactiae. NORTHEAST STRUCTURAL GENOMICS TARGET SaR32[1-108]." .

Assembly members:

Assembly members:
SaR32, polymer, 116 residues, 13226.012 Da.

Natural source:

Natural source: Common Name: Streptococcus agalactiae Taxonomy ID: 1311 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptococcus agalactiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: SaR32-1-108-21.3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SaR32: MMRLANGIVLDKDTTFGELK FSALRREVRIQNEDGSVSDE IKERTYDLKSKGQGRMIQVS IPASVPLKEFDYNARVELIN PIADTVATATYQGADVDWYI KADDIVLTLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	502
15N chemical shifts	117
1H chemical shifts	792

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SaR32	1

Entities:

Entity 1, SaR32 116 residues - 13226.012 Da.

C-terminal LEHHHHHH purification tag starting at residue 109. The construct was optimized by Hydrogen/Deuterium exchange mass spectrometry (DXMS)

1

MET

ARG

LEU

ALA

ASN

GLY

ILE

VAL

LEU

2

ASP

LYS

ASP

THR

PHE

GLY

GLU

LEU

LYS

3

PHE

SER

ALA

LEU

ARG

GLU

VAL

ARG

ILE

4

GLN

ASN

GLU

ASP

GLY

SER

VAL

SER

ASP

GLU

5

ILE

LYS

GLU

ARG

THR

TYR

ASP

LEU

LYS

SER

6

LYS

GLY

GLN

GLY

ARG

MET

ILE

GLN

VAL

SER

7

ILE

PRO

ALA

SER

VAL

PRO

LEU

LYS

GLU

PHE

8

ASP

TYR

ASN

ALA

ARG

VAL

GLU

LEU

ILE

ASN

9

PRO

ILE

ALA

ASP

THR

VAL

ALA

THR

ALA

THR

10

TYR

GLN

GLY

ALA

ASP

VAL

ASP

TRP

TYR

ILE

11

LYS

ALA

ASP

ILE

VAL

LEU

THR

LEU

GLU

12

HIS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC (aliph)	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY aliphatic	sample_1	isotropic	sample_conditions_1
CCH-TOCSY aliphatic	sample_1	isotropic	sample_conditions_1
3D simultaneous CN NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
simultaneous CN NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC (aliph)	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC high res. (L/V stereoassignment)	sample_3	isotropic	sample_conditions_1
2D 1H-15N hetNOE	sample_3	isotropic	sample_conditions_1
1D 1H-15N T1	sample_3	isotropic	sample_conditions_1
1D 1H-15N T2	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC (arom)	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC (arom)	sample_2	isotropic	sample_conditions_1

PDB	2K5D
DBJ	BAG12480 BAG80618 BAK28458 GAD37696
EMBL	CAQ49394 CAR69097 CAV31171 CAZ51616 CAZ55327
GB	AAB60011 AAM99819 AAY63928 ABP89898 ABP92096
PRF	2114402C
REF	NP_687947 WP_000985015 WP_001234286 WP_002843320 WP_016397616

BMRB Entry 15829

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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