BMRB Entry 15796
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PDB ID: 2k4d
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15796
MolProbity Validation Chart
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NMR-STAR v3 text file.
XML gzip file.
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Citation: Huang, Anding; De Jong, Rob; Wienk, Hans; Winkler, Sebastiaan; Timmers, Marc; Boelens, Rolf. "E2-c-Cbl recognition is necessary but not sufficient for ubiquitination activity" J. Mol. Biol. 385, 507-519 (2008).
PubMed: 18996392
Assembly members:
E2-c-Cbl, polymer, 83 residues, 9387.704 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pLICHISGST
Entity Sequences (FASTA):
E2-c-Cbl: GSLQDHIKVTQEQYELYCEM
GSTFQLCKICAENDKDVKIE
PCGHLMCTSCLTSWQESEGQ
GCPFCRCEIKGTEPIVVDPF
DPR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 403 |
| 15N chemical shifts | 93 |
| 1H chemical shifts | 590 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | E2-c-Cbl | 1 |
| 2 | ZINC ION_1 | 2 |
| 3 | ZINC ION_2 | 2 |
Entities:
Entity 1, E2-c-Cbl 83 residues - 9387.704 Da.
N-terminal GSL is a cloning artifact due to thrombin cleavage
| 1 | GLY | SER | LEU | GLN | ASP | HIS | ILE | LYS | VAL | THR | ||||
| 2 | GLN | GLU | GLN | TYR | GLU | LEU | TYR | CYS | GLU | MET | ||||
| 3 | GLY | SER | THR | PHE | GLN | LEU | CYS | LYS | ILE | CYS | ||||
| 4 | ALA | GLU | ASN | ASP | LYS | ASP | VAL | LYS | ILE | GLU | ||||
| 5 | PRO | CYS | GLY | HIS | LEU | MET | CYS | THR | SER | CYS | ||||
| 6 | LEU | THR | SER | TRP | GLN | GLU | SER | GLU | GLY | GLN | ||||
| 7 | GLY | CYS | PRO | PHE | CYS | ARG | CYS | GLU | ILE | LYS | ||||
| 8 | GLY | THR | GLU | PRO | ILE | VAL | VAL | ASP | PRO | PHE | ||||
| 9 | ASP | PRO | ARG |
Entity 2, ZINC ION_1 - Zn - 65.409 Da.
| 1 | ZN |
Related Database Links:
| PDB | 1FBV 2K4D 2Y1M 2Y1N 4A49 4A4B 4A4C |
| DBJ | BAC26087 BAE32253 |
| EMBL | CAA40393 |
| GB | AAF43710 AAG60692 AAH76671 AAH93450 AAI21225 |
| REF | NP_001006802 NP_001122110 NP_001245076 NP_005179 NP_031645 |
| SP | P22681 P22682 Q6DFR2 |
| TPG | DAA22315 |
| AlphaFold | P22681 P22682 Q6DFR2 |
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