BMRB Entry 15752

Name: Structure of a Glycosylphosphatidylinositol-anchored Domain from a Trypanosome Variant Surface Glycoprotein
Published: 2008-11-12

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PDB ID: 2jwh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15752
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of a Glycosylphosphatidylinositol-anchored Domain from a Trypanosome Variant Surface Glycoprotein

Deposition date:

2008-05-05

Original release date:

2008-11-12

Authors:

Jones, Nicola; Nietlispach, Daniel; Sharma, Reuben; Burke, David; Eyres, Isobel; Mues, Marsilius; Mott, Helen; Carrington, Mark

Citation:

Citation: Jones, Nicola; Nietlispach, Daniel; Sharma, Reuben; Burke, David; Eyres, Isobel; Mues, Marsilius; Mott, Helen; Carrington, Mark. "Structure of a glycosylphosphatidylinositol-anchored domain from a trypanosome variant surface glycoprotein" J. Biol. Chem. 283, 3584-3593 (2008).
PubMed: 18003615

Assembly members:

Assembly members:
Variant_Surface_Glycoprotein_ILTat1.24, polymer, 48 residues, 5141.820 Da.

Natural source:

Natural source: Common Name: Trypanosoma brucei Taxonomy ID: 5691 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trypanosoma brucei

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Variant_Surface_Glycoprotein_ILTat1.24: GTKASKSGVPVTQTQTAGAD TTAEKCKGKGEKDCKSPDCK WEGGTCKD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	276

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity	1

Entities:

Entity 1, entity 48 residues - 5141.820 Da.

Residue G1 represents a residual non-native residue from affinity tag cleavage. This is the second C-terminal subdomain of the Variant Surface Glycoprotein ILTat1.24

1

GLY

THR

LYS

ALA

SER

LYS

SER

GLY

VAL

PRO

2

VAL

THR

GLN

THR

GLN

THR

ALA

GLY

ALA

ASP

3

THR

ALA

GLU

LYS

CYS

LYS

GLY

LYS

GLY

4

GLU

LYS

ASP

CYS

LYS

SER

PRO

ASP

CYS

LYS

5

TRP

GLU

GLY

THR

CYS

LYS

ASP

Samples:

sample_1: ILTat1.24 C2-domain 0.5 mM; sodium phosphate 50 mM; sodium chloride 150 mM; sodium azide 0.05%; D2O 10%; H2O 90%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

AZARA, Boucher - processing

ANSIG, Kraulis - data analysis

ARIA, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

Bruker DRX 500 MHz

PDB	2JWH
EMBL	CAA39964 CAA40086
SP	P26329
AlphaFold	P26329