BMRB Entry 15744

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15744
MolProbity Validation Chart

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Title:
Backbone assignment of lipid-free human apolipoprotein E (apoE)
Deposition date:
2008-04-26
Original release date:
2008-10-15
Authors:
Zhang, Yonghong; Chen, Jianglei; Wang, Jianjun
Citation:

Citation: Zhang, Yonghong; Chen, Jianglei; Wang, Jianjun. "A complete backbone spectral assignment of lipid-free human apolipoprotein E (apoE)"  Biomol. NMR Assignments 2, 207-210 (2008).
PubMed: 19636906

Assembly members:

Assembly members:
Apolipoprotein_E, polymer, 308 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Apolipoprotein_E: MHHHHHHHHKVEQAVETEPE PELRQQTEWQSGQRWELALG RFWDYLRWVQTLSEQVQEEL LSSQVTQELRALMDETMKEL KAYKSELEEQLTPVAEETRA RLSKELQAAQARLGADMEDV CGRLVQYRGEVQAMLGQSTE ELRVRLASHLRKLRKRLLRD ADDLQKRLAVYQAGAREGAE RGLSAIRERLGPLVEQGRVR AATVGSLAGQPLQERAQAWG ERLRARMEEMGSRTRDRLDE VKEQVAEVRAKLEEQAQQIR LQAEAAQARLKSRFEPLAED MQRQWAGQVEKVQAAEGTSA APVPSDNH

Data sets:
Data typeCount
13C chemical shifts579
15N chemical shifts288
1H chemical shifts288

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Lipid-free human apolipoprotein E1

Entities:

Entity 1, Lipid-free human apolipoprotein E 308 residues - Formula weight is not available

1   METHISHISHISHISHISHISHISHISLYS
2   VALGLUGLNALAVALGLUTHRGLUPROGLU
3   PROGLULEUARGGLNGLNTHRGLUTRPGLN
4   SERGLYGLNARGTRPGLULEUALALEUGLY
5   ARGPHETRPASPTYRLEUARGTRPVALGLN
6   THRLEUSERGLUGLNVALGLNGLUGLULEU
7   LEUSERSERGLNVALTHRGLNGLULEUARG
8   ALALEUMETASPGLUTHRMETLYSGLULEU
9   LYSALATYRLYSSERGLULEUGLUGLUGLN
10   LEUTHRPROVALALAGLUGLUTHRARGALA
11   ARGLEUSERLYSGLULEUGLNALAALAGLN
12   ALAARGLEUGLYALAASPMETGLUASPVAL
13   CYSGLYARGLEUVALGLNTYRARGGLYGLU
14   VALGLNALAMETLEUGLYGLNSERTHRGLU
15   GLULEUARGVALARGLEUALASERHISLEU
16   ARGLYSLEUARGLYSARGLEULEUARGASP
17   ALAASPASPLEUGLNLYSARGLEUALAVAL
18   TYRGLNALAGLYALAARGGLUGLYALAGLU
19   ARGGLYLEUSERALAILEARGGLUARGLEU
20   GLYPROLEUVALGLUGLNGLYARGVALARG
21   ALAALATHRVALGLYSERLEUALAGLYGLN
22   PROLEUGLNGLUARGALAGLNALATRPGLY
23   GLUARGLEUARGALAARGMETGLUGLUMET
24   GLYSERARGTHRARGASPARGLEUASPGLU
25   VALLYSGLUGLNVALALAGLUVALARGALA
26   LYSLEUGLUGLUGLNALAGLNGLNILEARG
27   LEUGLNALAGLUALAALAGLNALAARGLEU
28   LYSSERARGPHEGLUPROLEUALAGLUASP
29   METGLNARGGLNTRPALAGLYGLNVALGLU
30   LYSVALGLNALAALAGLUGLYTHRSERALA
31   ALAPROVALPROSERASPASNHIS

Samples:

sample_1: Apolipoprotein E, [U-100% 13C; U-100% 15N; 80% 2H], 1 mM; phosphate buffer 25 mM; NaCl 25 mM; NaN3 0.05 mM; D2O 5%; DTT-d10 10 mM; EDTA 10 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.9; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAG37412 BAG72927
EMBL CAA25017
GB AAB59397 AAB59518 AAB59546 AAD02505 AAG27089
PRF 1506383A
REF NP_000032 NP_001289617 NP_001289618 NP_001289619 NP_001289620
SP P02649
AlphaFold P02649

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks