BMRB Entry 15735

Name: truncated AcrA from Campylobacter jejuni for glycosylation studies
Published: 2009-01-23

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PDB ID: 2k32
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15735
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

truncated AcrA from Campylobacter jejuni for glycosylation studies

Deposition date:

2008-04-17

Original release date:

2009-01-23

Authors:

Slynko, Vadim; Schubert, Mario; Numao, Shin; Kowarik, Michael; Aebi, Markus; Allain, Frederic

Citation:

Citation: Slynko, Vadim; Schubert, Mario; Numao, Shin; Kowarik, Michael; Aebi, Markus; Allain, Frederic. "NMR structure determination of a segmentally labeled glycoprotein using in vitro glycosylation" J. Am. Chem. Soc. 131, 1274-1281 (2009).
PubMed: 19154179

Assembly members:

Assembly members:
AcrA(61-210DD), polymer, 116 residues, 12748.376 Da.

Natural source:

Natural source: Common Name: Campylobacter jejuni Taxonomy ID: 197 Superkingdom: Bacteria Kingdom: not available Genus/species: Campylobacter jejuni

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AcrA(61-210DD): DVIIKPQVSGVIVNKLFKAG DKVKKGQTLFIIEQDQASKD FNRSKALFSQSAISQKEYDS SLATLDHTEIKAPFDGTIGD ALVNIGDYVSASTTELVRVT NLNPIYADGSHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	332
15N chemical shifts	117
1H chemical shifts	752

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity	1

Entities:

Entity 1, entity 116 residues - 12748.376 Da.

The construct consists of residues 61-210 of AcrA of C. jejuni with deletions of res. 97-117 and 146-166 and two mutations: K96Q K131Q.

1

ASP

VAL

ILE

LYS

PRO

GLN

VAL

SER

GLY

2

VAL

ILE

VAL

ASN

LYS

LEU

PHE

LYS

ALA

GLY

3

ASP

LYS

VAL

LYS

GLY

GLN

THR

LEU

PHE

4

ILE

GLU

GLN

ASP

GLN

ALA

SER

LYS

ASP

5

PHE

ASN

ARG

SER

LYS

ALA

LEU

PHE

SER

GLN

6

SER

ALA

ILE

SER

GLN

LYS

GLU

TYR

ASP

SER

7

SER

LEU

ALA

THR

LEU

ASP

HIS

THR

GLU

ILE

8

LYS

ALA

PRO

PHE

ASP

GLY

THR

ILE

GLY

ASP

9

ALA

LEU

VAL

ASN

ILE

GLY

ASP

TYR

VAL

SER

10

ALA

SER

THR

GLU

LEU

VAL

ARG

VAL

THR

11

ASN

LEU

ASN

PRO

ILE

TYR

ALA

ASP

GLY

SER

12

HIS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_3	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HN(CO)CA	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

BMRB Entry 15735

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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