BMRB Entry 15720

Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15720

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Title:
Solution structure of Arabidopsis thaliana protein At1g70830, a member of the major latex protein family
Deposition date:
2008-04-07
Original release date:
2008-06-03
Authors:
Volkman, B.; de la Cruz, N.; Lytle, B.; Peterson, F.
Citation:

Citation: Lytle, Betsy; Song, Jikui; de la Cruz, Noberto; Peterson, Francis; Johnson, Kenneth; Bingman, Craig; Phillips, George; Volkman, Brian. "Structures of two Arabidopsis thaliana major latex proteins represent novel helix-grip folds"  Proteins 76, 237-243 (2009).
PubMed: 19326460

Assembly members:

Assembly members:
major_latex_protein-like_protein_28_or_MLP-like_protein_28, polymer, 166 residues, 18682.152 Da.

Natural source:

Natural source:   Common Name: Mouse-ear cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: wheat germ - cell free   Vector: pEU-HIS

Entity Sequences (FASTA):

Entity Sequences (FASTA):
major_latex_protein-like_protein_28_or_MLP-like_protein_28: GHHHHHHLETEASSLVGKLE TDVEIKASADKFHHMFAGKP HHVSKASPGNIQGCDLHEGD WGTVGSIVFWNYVHDGEAKV AKERIEAVEPDKNLITFRVI EGDLMKEYKSFLLTIQVTPK PGGPGSIVHWHLEYEKISEE VAHPETLLQFCVEVSKEIDE HLLAEE

Data sets:
Data typeCount
13C chemical shifts665
15N chemical shifts156
1H chemical shifts1047

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1major latex protein-like protein 28 or MLP-like protein 281

Entities:

Entity 1, major latex protein-like protein 28 or MLP-like protein 28 166 residues - 18682.152 Da.

1   GLYHISHISHISHISHISHISLEUGLUTHR
2   GLUALASERSERLEUVALGLYLYSLEUGLU
3   THRASPVALGLUILELYSALASERALAASP
4   LYSPHEHISHISMETPHEALAGLYLYSPRO
5   HISHISVALSERLYSALASERPROGLYASN
6   ILEGLNGLYCYSASPLEUHISGLUGLYASP
7   TRPGLYTHRVALGLYSERILEVALPHETRP
8   ASNTYRVALHISASPGLYGLUALALYSVAL
9   ALALYSGLUARGILEGLUALAVALGLUPRO
10   ASPLYSASNLEUILETHRPHEARGVALILE
11   GLUGLYASPLEUMETLYSGLUTYRLYSSER
12   PHELEULEUTHRILEGLNVALTHRPROLYS
13   PROGLYGLYPROGLYSERILEVALHISTRP
14   HISLEUGLUTYRGLULYSILESERGLUGLU
15   VALALAHISPROGLUTHRLEULEUGLNPHE
16   CYSVALGLUVALSERLYSGLUILEASPGLU
17   HISLEULEUALAGLUGLU

Samples:

sample_1: major latex protein-like protein 28 or MLP-like protein 28, [U-100% 13C; U-100% 15N], 0.7 mM; DTT 5 mM; Bis-Tris, [U-100% 2H], 10 mM; D2O 5%; H2O 95%

sample_conditions_1: ionic strength: 0.002 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYsample_1isotropicsample_conditions_1
3D_13C-separated_NOESYsample_1isotropicsample_conditions_1
3D_13C-separated_NOESY (AROMATIC)sample_1isotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v2004, Delagio,F. et al. - processing

XEASY v1.3, Eccles, C.; Guntert, P.; Billeter, M.; Wuthrich, K. - data analysis

SPSCAN v1.1.0, R.W. Glaser - data analysis

GARANT v2.1, C. Bartels - data analysis

CYANA v2.1, Guntert, P. - structure solution

X-PLOR NIH v2.9.3, SCHWIETERS, C.D., KUSZEWSKI, J.J., TJANDRA, N., CLORE, G.M. - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
GB AEE35123 AEE35125
REF NP_001077806 NP_001117579

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks